Zymogen agent for treating agricultural wastes and preparation method
A technology for agricultural waste and fermentation inoculum, applied in the field of fermentation inoculum and its manufacture, can solve the problems of insufficient fermentation, low fermentation temperature, and incomplete killing of harmful organisms.
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[0042] 1. Preparation of yeast strain ZJY2:
[0043] Saccharomyces spp. ZJY2 liquid shake flask medium: wort juice (10·Bx) 1000ml, glucose 18g, pH natural. Add 20 grams of agar with this as the medium to prepare a solid mother seed slant for inoculation and culture, take a ring of the slant mother seed and inoculate it in the culture medium of the yeast liquid shake flask (60ml medium / 250ml Erlenmeyer flask) according to the aseptic method , 28°C, rotation speed 180rpm, cultivated for 18 hours, the number of bacteria reached 0.5×10 8 More than cfu / ml is enough. Saccharomyces spp. solid fermentation culture medium: 99% of bran and a small amount of inorganic salt MgSO 4 , NaCl each 0.05%, according to material, water ratio 1: 1 (w / w) add water to make material water content be about 60%. Take the liquid microbial agent cultivated in shake flasks as the bacterial classification, and evenly inoculate it in the solid fermentation material koji with an inoculation amount of 0.1%...
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