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Method for increasing anti-sun light radiation of biological pesticide

An anti-sunlight and biological pesticide technology, applied in botanical equipment and methods, biocides, animal repellents, etc. The effect of improving the irradiation power

Inactive Publication Date: 2006-09-20
UNIV OF SCI & TECH BEIJING
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AI Technical Summary

Problems solved by technology

Sunlight has little effect on the activity of spores and exotoxins, but parasporal crystals are easy to lose activity, which is the primary factor affecting its field control effect
Pesticides, 1998, 37(7): 16-17 reported the use of starch-wrapped Bt to resist sunlight. Due to the low strength of the wrapped shell and the lack of bonding force between starch and Bt, it was used in the field. The wrapping is highly prone to cracking, which is not as effective as it should be
The Journal of Natural Sciences of Hunan Normal University, 2001, 24(1): 66-68 introduces the compounding of Cordyceps Gurni and Bt to increase photostability, but the adsorption between Cordyceps Gurni and Bt is very small, and during use It is easy to separate in the middle and lead to little effect

Method used

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  • Method for increasing anti-sun light radiation of biological pesticide
  • Method for increasing anti-sun light radiation of biological pesticide

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Embodiment Construction

[0013] Activation and inoculation on the slope of the solid medium, amplification of the bacteria in the liquid seed medium, at a speed of 120r / min, control the temperature at 30°C, and cultivate for 20 hours to obtain the Bt fermentation broth;

[0014] Take the Bt fermentation broth, add p-aminobenzoic acid according to 1% of the weight of the Bt fermentation broth, and stir at 20°C for 40 minutes to make it fully react;

[0015] Take the above reaction solution, add clay according to one-third of its weight, stir for 20 minutes, let it settle, filter, dry at 50°C, and pulverize to obtain the product.

[0016] The insecticidal potency of the product is 16000IU / mg. If it is irradiated with simulated sunlight (30 watts, 200nm-1000nm) for 300 minutes, its insecticidal potency will become 8000IU / mg, that is, 300 minutes of irradiation can make its potency 50% reduction. It can be seen that the method can significantly improve the anti-sunlight irradiation ability of the Bt biol...

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Abstract

The present invention relates to preparation of biological pesticide. Bacillus thringiensis fermenting liquid obtained through activating inoculation, culture and magnifying is added with compound containing -NH4 and / or -COOH as protecting agent in the amount of 10 ppm to 1% and white clay in the amount of 1-33%. The Bacillus thringiensis parasporal crystal is connected to the protecting agent via chemical bond, and the protecting agent absorbs the energy of sun light to protect the parasporal crystal. The product can resist sun irradiation with the resisting wavelength being 200-1000 nm.

Description

technical field [0001] The invention relates to the preparation of a biological pesticide, which belongs to the international patent classification A01N63 / 02. Background technique [0002] Bacillus Thuringiensis (Bt) has become a dominant biopesticide. It has a good effect on preventing pests and diseases, is safe and non-toxic to humans and animals, does not kill natural enemies and beneficial organisms of pests, and has low environmental risk. It pollutes the environment, has no residue, and it is difficult for pests and disease sources to develop resistance. It has achieved remarkable economic, social and ecological benefits. However, it has three disadvantages of slow drug effect, high production cost and poor photostability. In order to overcome its shortcomings, scientists have made unremitting efforts. The study of genetic engineering has greatly broadened its insecticidal spectrum and improved its efficacy; improving the production process and using MSG waste liqui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02
Inventor 弓爱军邱丽娜温美娟
Owner UNIV OF SCI & TECH BEIJING
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