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Purification method of protoheme

A purification method and heme technology, applied in the field of heme purification, can solve the problems of high production cost, environmental pollution, high product price, etc., and achieve the effect of low cost and convenient industrial production.

Inactive Publication Date: 2006-10-18
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The heme extraction process mainly contains sodium acetate method, distillation method, tannic acid method, carboxymethyl cellulose method, glacial acetic acid method, acid acetone method, also once adopted the esterification method to test in the prior art, but most methods Can only prepare heme crude product (content is below 80%), further purify heme, make heme content higher than 96%, basically cannot do without the use of organic solvent in these methods, thereby cause high production cost, serious environmental pollution , resulting in high product prices, the current market price of 98% hemoglobin is 10,000-12,000 yuan / kg

Method used

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  • Purification method of protoheme

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Experimental program
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Effect test

Embodiment 1

[0021] Take 1kg of heme with 80% content, add 25L of 2.0% dilute ammonia solution, stir for 15 minutes, centrifuge to remove the precipitate, then add 7.5% hydrochloric acid to the solution to adjust the pH value to about 3, after flocculent precipitate appears, centrifuge to remove the precipitate The precipitate was repeatedly washed with water until neutral, and dried to obtain a pure product, which was analyzed to have a purity of 98%.

Embodiment 2

[0023] Take 1 kg of 80% heme, add 50 L of 1.0% dilute sodium hydroxide solution, stir for 10 minutes, centrifuge to remove the precipitate, then add 5% hydrochloric acid to the solution to adjust the pH value to about 3, after flocculent precipitation appears, centrifuge The precipitate was taken, washed with water repeatedly until neutral, and dried to obtain a pure product, which was analyzed to have a purity of 98%.

Embodiment 3

[0025] Take 1kg of heme with 80% content, add 10L of 3.0% dilute potassium hydroxide solution, stir for 5 minutes, centrifuge to remove the precipitate, then add 10% hydrochloric acid to the solution to adjust the pH value to about 3, after flocculent precipitation appears, centrifuge The precipitate was taken, washed repeatedly with water until neutral, and dried to obtain a pure product, which was analyzed to have a purity of 98%.

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Abstract

A process for purifying heme includes such steps as mixing raw heme with diluted alkali solution, stirring, centrifugal separation of deposit, adding diluted acid solution, educing heme, centrifugal separation of deposit, regulating pH value to make it neutral and drying. Its advantages are high purity (more than 96%), low cost and no pollution.

Description

technical field [0001] The invention relates to a purification technology of heme, in particular to a method for purifying heme by utilizing the property of different solubility of heme in different pH solutions. Background technique [0002] Heme (Heme, Hame, Protoheme) in animals is usually hemoglobin (Hemoglobins), invertebrate hemoglobin (Erythrocrorins), myoglobin (Myoglobins), cytochrome b (Cytochrome b) and some enzymes with biocatalysis such as The prosthetic group of peroxidase, catalase, and cytochrome COxidase is the color-furnishing component of hemoglobin. [0003] In the blood, it exists in the red blood cells (Erythrocyte) and combines with globin, the component of hemoglobin, in a non-covalent bond. The color of blood is also caused by the color of hemoglobin. [0004] Heme is an iron-containing compound, a polyheterocyclic compound composed of four pyrrole rings through four carbon atoms, called porphine, and the derivatives of porphine are also called porp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F15/02
Inventor 周淡宜王茵徐水祥朱染枫
Owner ZHEJIANG ACAD OF MEDICAL SCI
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