Preparation method of Chinese medicinal agent possessing right supporting anticancer function and its application
A technology of injections and traditional Chinese medicines, applied in medical preparations containing active ingredients, antineoplastic drugs, drug combinations, etc., can solve problems such as limiting the therapeutic effect of Fuzheng prescriptions
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Embodiment 1
[0081] Embodiment 1, the preparation 1 of composition total extract
[0082] Take the coarse powder of the following medicinal materials: Fufangteng 334g, Astragalus membranaceus 167g, red ginseng 20g, put in a round bottom flask, add 2000ml, 30% ethanol solution, heat reflux extraction, extract 3 times, each 40min; 1000ml of deionized water, heated to reflux for extraction for 60min. Combine all the extracts, evaporate the ethanol, and concentrate to about 500ml; add an appropriate amount of chitosan, and remove impurities by flocculation; extract the extract after removing impurities with n-butanol, and extract 300ml of n-butanol pre-saturated with water for 5 times , combine the organic phases, concentrate, evaporate n-butanol, dissolve with an appropriate amount of ethanol, add activated carbon, reflux for 15 minutes, decarburize, and dry to obtain the medium and low polarity small molecule extract; take the remaining aqueous phase of the extraction, ultrafilter, Enrich s...
Embodiment 2
[0083] Embodiment 2, the preparation 2 of composition total extract
[0084] Take the following medicinal materials: fufangteng 10g, ginseng 100g, astragalus 500g, grind them into coarse powder, and set aside.
[0085] Take Astragalus membranaceus and Fufangteng, extract with 1000ml of deionized water, heat reflux extraction, 60min each time, extract 3 times, combine all extracts, concentrate to 600ml, pass the concentrated solution through DIAION HP 20 macroporous adsorption resin column, 3 times Rinse with water of the column bed volume, collect the water eluent, and set aside; then elute with 80% ethanol solution, collect 3 times the column bed volume of the eluent, concentrate, and dry, that is, the medium and low polarity small molecule extraction matter (E.g); take the water eluate, put it into a semi-permeable membrane, fully dialyze, remove the protein from the macromolecular part, and obtain the E.s part.
[0086] Take ginseng, extract with 40% methanol solution, hea...
Embodiment 3
[0088] Embodiment 3, composition total extract preparation 3
[0089] Take the following medicinal materials: fufangteng 1000g, ginseng 10g, astragalus 10g, pulverize and set aside.
[0090] Take the coarse powder of Fufangteng medicinal material, add 2000ml of 50% ethanol solution, heat reflux extraction, 50min each time, extract twice; then extract with 1000ml deionized water for 30min, combine all extracts, evaporate ethanol, concentrate to about 1000ml, Stand still, remove the precipitate, take the clarified extract, add an appropriate amount of flocculant for treatment, remove the flocculant, pass the extract through D101 type adsorption resin, and elute with deionized water of 3 times the volume of the column bed, collect the washing liquid, and set aside; Elute with 80% ethanol to obtain small molecule extracts, and decolorize with an appropriate amount of activated carbon to obtain the product. Concentrate the washing solution to 200ml, add 10 times the volume of etha...
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