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Sporolactobacillaceae and produced preparation of living fungus thereof

A technology of Lactobacillus sporogenes and bacterial agents, which is applied in the direction of bacteria, animal feed, animal feed, etc., can solve the problems of low production and use costs, and achieve the effects of low production costs, reduced diarrhea rate, and reduced pig raising costs

Inactive Publication Date: 2007-06-20
NANJING AGRICULTURAL UNIVERSITY
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  • Description
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Problems solved by technology

At the same time, the production and use costs are lower

Method used

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  • Sporolactobacillaceae and produced preparation of living fungus thereof

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Embodiment Construction

[0022] 1 Isolation and identification of strains

[0023] Take intestinal chyme and intestinal wall of piglets aseptically, dissolve them in sterile saline, and mix the diluted solution with a vortex mixer, 1000 r.min -1 After centrifugation, the supernatant was serially diluted and inoculated on solid medium, and cultured at 37°C for several days. Several colonies were picked from them, and the results of strain optimization showed that the growth rate of S1 bacteria was extremely fast, and it could reach a plateau 10 hours after inoculation, as shown in Figure 3; S1 bacteria are resistant to low pH, high temperature, high bile salt concentration and inhibition Escherichia coli and Staphylococcus aureus, identified as Sporolaciobacillus sp., had the best results. The main biological characteristic is G + , the thallus is slender and rod-shaped, with segments, oval spores, spores are obvious after malachite green staining, facultative anaerobic growth. The bacterium can be ...

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Abstract

The present invention provides a kind of bacterium and its procreation of living bacterium reagent, which can reduce the rate of piglet diarrhea. The used of bacterium is gelanshi pigmentation feedback electropositive bacterium S1, according to appraisal, it is Sporolactobacillus sp..Its main biological character is G+, the shape of bacterium is slim, divided burl, elliptical sporangium, after malachite pigmentation pigmentation is obvious, concurrently, exclude oxygen development, endure lower pH(pH2.5) and higher saline chroma(0.2%), not sensetive to pepsin, endure high temperature, it can restrain coliform and golden ball bacterium well. It indicated from the track of molecule biological technology, this bacterium can propagated in alvine of piglet. The flow chart is bevel bacterium--- rocky bottle seed liquid--- ferment jar---freez dryness--- production. The animal test indicates, this bacterium can be used directly and widely in reduce alvine of piglet, enhance the growth rate of piglet, reduce the use of antibiotics in piglet feedstuff.

Description

1. Technical field [0001] A kind of Lactobacillus sporogenes S1 of the present invention and the bacterial agent produced thereof belong to the field of biological high technology, and are used to reduce the diarrhea rate of piglets and improve the growth rate of piglets by adjusting the microecology of the intestinal tract of animals, and are suitable for modern agriculture The production of green and pollution-free livestock products in production. 2. Technical background [0002] Since the U.S. Food and Drug Administration (FDA) first approved antibiotics as feed additives in the 1950s, countries around the world have successively carried out feeding experiments on antibiotics and used them in production. Undoubtedly, antibiotics have played a positive role in preventing animal diseases and promoting animal growth as growth-promoting feed additives. But over time and as science has advanced, it has been recognized that the use of antimicrobials such as feed antibiotics i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/00A23K1/18A23K10/18A23K50/30A23K50/60
Inventor 朱伟云姚文毛胜勇
Owner NANJING AGRICULTURAL UNIVERSITY