Fast detecting method for aftatoxin B*

Abstract

The present invention is fast detection method of aflatoxin B1 in food and feed. The fast detection method includes the following steps: adding ground sample to be tested in 2-10 g in test tube, adding methanol-sodium chloride aqua of 50-80 % concentration in 10-25 ml, ultrasonic extraction in water bath at 40-60 deg.c, and suction filtering to obtain filtrate in 4-10 ml; adding re-distilled petroleum ether in 2-4 ml into the filtrate, shaking, letting stand to laminate, taking the lower layer of solution in 1-4 ml and adding pure water in 4-20 ml; adding to micro immunoaffinity column of aflatoxin B1, washing with methanol aqua of 10-40 % concentration in 5-10 ml, eluting with methanol in 0.5-2.0 ml, collecting the eluted liquid, adding test agent A in 0.2-1.0 ml; and detecting in fluorescent spectrophotometry or similar instrument. The present invention has the features of simple process, high detection precision and stability, fast detection speed and high safety.

Description

technical field [0001] The present invention relates to a kind of aflatoxin B in food and feed 1 quick test method. Background technique [0002] Mycotoxins are secondary metabolites secreted by toxin-producing fungi, and are natural toxic compounds that can cause various damages to humans and animals. Among the mycotoxins that have been discovered, aflatoxin B 1 (aflatoxin B 1 , referred to as AFB 1 ) is the most toxic mycotoxin, and its toxicity, carcinogenicity and pollution frequency all rank first in biological toxins. After it pollutes food and feed, it directly or indirectly enters the human food chain, threatening human health and life safety, and the degree of harm is proportional to the intake of aflatoxin. Aflatoxin widely exists in agricultural products such as rice, corn, peanuts, sesame, soybeans, rapeseed, fish and other foods, and there are many links in which it contaminates food and feed. 1 The maximum allowable content in food and feed is used as a m...

AI Technical Summary

Problems solved by technology

Among them, thin-layer chromatography is the most commonly used detection method for detecting aflatoxins. It does not require special equipment and can be carried out in general laboratories, but the amount of reagents is large, the operation is cumbersome, the interference of other components is serious, and the accuracy is poor. Quantitative, and more harmful to experimenters and the environment, not suitable for on-site rapid detection

Precision instrument analysis methods include fluorescence spectrophotometry and high performance liquid chromatography, which have high sensitivity and good accuracy, but the instruments are expensive, requiring a high degree of purification of aflatoxin samples, and the sample pretreatment process is cumbersome and time-consuming. High, difficult to achieve rapid detection

Immunological analysis methods include enzyme immunoassay, radioimmunoassay and time-resolved fluorescence method. Enzyme immunoassay has the advantages of strong specificity, high sensitivity, low cost, and is suitable for batch detection. However, it is generally a qualitative or semi-quantitative method. The detection of aflatoxins in agricultural products with complex components is susceptible to interference, and there are disadvantages such as a high proportion of false positives and low detection accuracy

Radioimmunoassay is similar to enzyme immunoassay, but radioimmunoassay still has radioactive pollution, which is likely to bring additional adverse effects on operators and the environment, and is rarely used now

Time-resolved fluorescence method has been applied in recent years, and its detection sensitivity is higher than the former two, but the pre-processing is still complicated, and the required equipment is expensive, so it is limited to the use in the field of medical clinical diagnosis

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