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Electrochemical detector

A detector and electrochemical technology, applied in the field of biological detectors, can solve the problem of high analyte concentration estimation

Inactive Publication Date: 2003-01-01
LIFESCAN INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These effects can bias the estimate of analyte concentration

Method used

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  • Electrochemical detector
  • Electrochemical detector
  • Electrochemical detector

Examples

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example 1

[0072] The film is 130 microns thick and covered with a 60 nm thick layer of platinum. The area was defined by compressing the membrane to be 12.6 square millimeters. A 1.5 ml solution containing 0.2 molar potassium ferricyanide and 1% by weight glucose oxidase dehydrogenase was introduced into the volume defined by the membrane and allowed to evaporate the water.

[0073] The platinum layer was then connected to a potentiostat to serve as the working and counter / reference electrode. 3.0 ml of a clear solution containing 5 mM D-glucose and 0.9 wt% NaCl was dropped on a defined area of ​​the membrane. After 20 seconds, a voltage of 300 mV was applied between the working electrode and the counter / reference electrode and the current was recorded at 0.1 second intervals for the next 30 seconds.

[0074] Figure 13 is the curve of current versus time according to the above measurements. The function ln(i / 26.9-1) was calculated using a steady state current of 26.9 mA and plotted...

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Abstract

There is described a hollow electrochemical cell comprising a working electrode (5), a counter electrode (6), and an opening (31) for admitting an analyte to the cell, the working electrode (5) being spaced from the counter electrode (6) by less than 500 mu m.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 96194874.4 with a filing date of June 19, 1996. technical field [0002] The present invention relates to a biodetector, in particular to an electrochemical biodetector for determining the concentration of an analyte in a carrier fluid. The present invention is particularly applicable to the determination of glucose concentration in blood, and is described herein as such, but it should be understood that the present invention can also be used for the determination of other analytes. Background technique [0003] Electrochemical biodetectors generally include an electrolytic cell with a working electrode, a counter electrode, and a reference electrode. Sometimes the functions of the counter electrode and the reference electrode are combined into one electrode, called the "counter / reference" electrode or "quasi-reference electrode". As used herein, the term "counter electrode" generally incl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327C12Q1/00G01N27/416G01N27/42G01N27/49G01N33/487
CPCC12Q1/004G01N27/3273C12Q1/006
Inventor 阿拉斯泰尔·迈克英多·霍奇斯托马斯·威廉·贝克奥德瓦·乔纳森
Owner LIFESCAN INC