Staining kit and preparation method, staining method, and use thereof

Abstract

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

Description

(1) Technical field:

[0001] The invention relates to a staining reagent kit, a preparation method thereof, a staining method and the application in rapid multi-pathogen and cytological examination of reproductive tract infections / sexually transmitted diseases. (two) background technology:

[0002] The traditional staining methods are Papanicolaou and HE, which are world-renowned staining methods for cytopathology. The fixation time is long, the staining time is long, the staining steps are many, the reporting speed is slow, the staff is large, the number of daily readings is small, and the cost is high. The operation is complicated, labor-intensive, time-consuming and expensive.

[0003] Giemsa rapid staining method. Bryon-PA (Patnol Biol 9aris 1996; 24(1): 75-9) once reported that Giemsa rapid staining method was used for bone marrow smear examination. In 1977, Thatcher-RW and others were used for conjunctival cytological examination, and in 1978, Howell-WM (Hum Genet. 19...