Microfluid biological sensor chip device and its application

A biosensor, fluid biology technology, applied in the field of biosensors, to achieve the effect of wide application range, wide adaptability and convenient use

Inactive Publication Date: 2003-12-10
INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Microfluid biological sensor chip device and its application
  • Microfluid biological sensor chip device and its application
  • Microfluid biological sensor chip device and its application

Examples

Experimental program
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Embodiment

[0034] Embodiment: the mensuration of 1 formaldehyde pollution environment gas:

[0035] Basic principle: on the filterable water-absorbing material (fixed bracket, cellulose (No. 1) filter paper, diameter 6mm), and adsorb polylysine on the front of the filter (5ul / each piece, polylysine acid concentration 0.01 mg / ml) after drying, baked at 90° C. for three hours, and firmly fixed on filter paper with photosynthetic complexes (Chromatophores) (protein complexes). In order to maintain the active function of the photosynthetic complex, the filter paper material is loaded into a sandwich, and the surface containing functional molecules is the front of the chip, facing the direction of the sample inlet to be tested, and the back is in contact with the polytetrafluoroethylene filter. combination. The solution is filled between the functional biochip and the bilayer of the polytetrafluoroethylene sheet, in order to keep the biosensor chip to provide a solution, so that the bios...

Embodiment 2

[0049] Embodiment 2: the mensuration of influenza virus antibody antigen

[0050] 1. Materials: Influenza virus A (shang hai / 7 / 99) was isolated from influenza patients, inoculated and multiplied in the allantoic cavity of 9-10d-old chicken embryos, and the fresh allantoic fluid harvested was the experimental virus. The above-mentioned strains were cultivated in the virus culture medium and immunized by inoculated SPF chickens to prepare polyclonal antibodies of the susceptible virus.

[0051] 2. The pretreatment of the chip is as follows: After soaking the NC membrane with 20% methanol 20mM phosphate solution 50mM pH7.4 for two hours, wash it three times with 20mM phosphate solution 50mM pH7.4, and use ProteinA1mg / ml 100ul carbonate solution 50mM pH 9.6, overnight at 4°C, add 10ug of polyclonal antibody per well at 4°C overnight, then block with 10% BSA overnight.

[0052] 3. Fluorescence technology: take out the chip and measure it on a fluorescence spectrometer, measu...

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Abstract

The present invention uses the replaseable biosensor chip technology as core, and uses the related MEMS system formed from microreactor and microflow cell to make determination of environment pollution. After the harmfule molecule (for example influenza virus or SARS virus) is combined on the functional chip, said polluted molecule can store the information, and can release it in specific environment, and its released information is directly propertional to the polluted extent, so that it can implement the determination of environmental pollution.

Description

Technical field: [0001] The invention belongs to the technical field of biosensors. Specifically, it will involve a microsystem that uses biochips with different functions to detect different pollution sources in the environment. The detection system of the invention can be used for effective detection of chemical molecule (poison) gas and biological macromolecules in the environment, such as virus molecules and pathogenic bacteria. technical background: [0002] Urban residents spend 70% of their time indoors, and the conditions of the indoor environment are closely related to people's health. At present, although there are advanced large-scale instruments such as HPLC and mass spectrometry in the measurement technology of environmental pollution at home and abroad, for the measurement of a large amount of environmental pollution, the use of large-scale instruments is not only costly, but also produces a large amount of organic waste water during the measurement process. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64G01N21/76G01N33/00G01N33/53G01N33/543G01N33/68
Inventor 乐加昌崔元波孟涛徐红
Owner INSITUTE OF BIOPHYSICS CHINESE ACADEMY OF SCIENCES
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