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Prepn process of sulfogalacto oligose with antitumor activity

A technology for galacto-oligosaccharide and anti-tumor activity is applied in the field of preparation of sulfated galacto-oligosaccharide, and can solve the problems of unstable product quality, easily damaged active groups, severe reaction conditions, etc. The effect of simple preparation process and high product yield

Inactive Publication Date: 2004-04-07
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

[0002] At present, there are reports on the preparation of sulfated galactooligosaccharides by chemical methods, but this method has disadvantages such as severe reaction conditions, easily damaged active groups, and unstable product quality.

Method used

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Embodiment Construction

[0006] Dissolve 2g of κ-carrageenan in 100ml of distilled water, add 5 units of κ-carrageenase powder produced by marine cellophages, and 1 unit of enzyme activity is defined as the production of 1μmol reducing sugar per minute at 32℃. Galactose) The amount of enzyme required. The reaction was shaken at 32°C at 150r / min for 12h, heated in a boiling water bath to inactivate the enzyme, cooled and centrifuged at 3000r / min for 7min to remove insoluble fragments that were not enzymatically hydrolyzed. Add 1 volume of ethanol to the supernatant to remove large molecular weight. The remaining soluble components were concentrated by rotary evaporation at 60°C to 20ml, then 3 times the volume of ethanol was added to precipitate the oligosaccharides, the precipitate was collected by centrifugation, and dried at 70°C to obtain carrageenan enzymatic fragments. Under ice-salt bath conditions, add 5ml of formamide to the three-necked flask and keep stirring. Use a constant pressure separatory ...

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Abstract

The preparation process of sulfogalacto oligose with antitumor activity includes compounding kappa-carrageenan substrate, adding kappa-carrageenan enzyme powder for reaction, boiling water bath heating to deactivating enzyme, centrifuging to eliminate enzymolyzed resistance segment, ethanol precipitaition to collecting soluble enzymolyzed oligose, concentration, drying, sulfonating to form derivative, repeated ethanol washing, vacuum filtering and drying. The present invention features the kappa-carrageenan enzyme marine microbe produces, and the themical modification of the enzymolyzed oiligose after concentration and drying. The combination of enzymolysis degrading and chemical modification results in simple preparation process, high yield of product, stable quality and no damage of active radical, and the prouduct has antitumor activity and latent capacity of developing antitumor medicine.

Description

Technical field [0001] The invention relates to an organic polymer, in particular to a method for preparing galacto-oligosaccharides with anti-tumor activity. Background technique [0002] At present, it has been reported that a chemical method is used to prepare galacto-oligosaccharides, but this method has the disadvantages of severe reaction conditions, easy destruction of active groups, and unstable product quality. Summary of the invention [0003] The purpose of the present invention is to provide a method for preparing galacto-oligosaccharides with anti-tumor activity, which can overcome the above-mentioned shortcomings of the prior art. [0004] A method for preparing galacto-oligosaccharides with anti-tumor activity includes preparing a κ-carrageenan substrate, adding κ-carrageenase powder, heating in a boiling water bath after the reaction to inactivate the enzyme, and centrifugation to remove the enzymatic antibody The soluble fragments were precipitated with ethanol ...

Claims

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Application Information

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IPC IPC(8): A61P35/00C07H3/06C12P19/00
Inventor 牟海津江晓路管华诗
Owner OCEAN UNIV OF CHINA
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