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High-efficient soluble expression vector system universal for colibacillus

A technology of expression vector and Escherichia coli, which is applied in the field of genetic engineering and can solve problems such as unsatisfactory results

Inactive Publication Date: 2004-06-09
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1
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  • Summary
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  • Description
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Problems solved by technology

However, although GroEL / ES, which only has the function of a molecular chaperone, has a solubility-promoting effect on some proteins in practical applications, it has not achieved ideal results for many foreign proteins.

Method used

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  • High-efficient soluble expression vector system universal for colibacillus
  • High-efficient soluble expression vector system universal for colibacillus
  • High-efficient soluble expression vector system universal for colibacillus

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Embodiment

[0034] Specific primers of appropriate size were designed, and the complete coding sequence of Escherichia coli folding-related protein FkpA was amplified by polymerase chain reaction (PCR) using the genomic DNA of common Escherichia coli strain K12 as a template, but without the stop codon. The amplified product is recovered, digested and connected to the vector plasmid pTMF to obtain the vector pTFA which can be used for fusion expression. The fragment containing FkpA was excised from pTFA with endonucleases BglII and SacI, and connected to the vector containing anti-bladder cancer single-chain antibody, and a fusion expression vector for highly soluble expression of anti-bladder cancer single-chain antibody was constructed pTFP1. In order to construct the co-expression vector, a pair of specific primers for FkpA was additionally designed, in which the upstream primers contained the Escherichia coli translation recognition sequence-ribosome binding site (RBS), so as to facil...

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Abstract

The present invention relates to a novel universal carrier capable of high-effectively expressing exogenous protein in the colibacillus and in the soluble form. This universal carrier is a fusion and co-expression carrier established on the basis of colibacillus stimulated folding related protein FkpA; Said invention also relates to an expression carrier pTFP1 for high-effectively soluble-expressing single chain antibody (PG) for resisting carcinoma of bladder and expression carrier for high-effectively soluble-expressing fusion protein CBD-PG formed from cellulose binding domain (CBD) and PG; and a host cell containing above-mentioned universal high-effective soluble expression carrier.

Description

technical field [0001] The present invention relates to the field of genetic engineering, and more specifically, relates to a novel universal vector capable of highly expressing foreign proteins in a soluble form in Escherichia coli; a method for constructing the expression vector; and a host cell containing the vector. technical background [0002] Escherichia coli has many unique advantages as an expression system for exogenous proteins. For example, E. coli has a clear genetic background, rapid cell growth, short fermentation cycle, easy screening and operation, and abundant expression systems. However, a problem that cannot be ignored is that many foreign proteins expressed in E. coli aggregate in an inactive form, which is the inclusion body. Starting from inclusion bodies, denaturation, refolding and renaturation under certain conditions are necessary to obtain the desired active protein product. The conditions required for these processes often need to be optimized f...

Claims

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Application Information

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IPC IPC(8): C12N15/62C12N15/67C12N15/70
Inventor 张众黄华樑王飞宋丽萍周志勇林晴
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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