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Enzyme-linked immunosorbent assay kit for analyzing residual Carbaryl

An enzyme-linked immunosorbent assay, carbaryl technology, used in analytical materials, biological tests, measurement devices, etc.

Inactive Publication Date: 2004-08-25
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, such as Chinese invention patent publications CN1431503A and CN1431501A, all disclose kits for the detection of various common pesticide residues or metabolites that are troublesome for detection, including detection of residues such as methyl parathion and thion Kit and preparation method thereof
And there is no technology about conveniently detecting carbaryl in the prior art

Method used

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  • Enzyme-linked immunosorbent assay kit for analyzing residual Carbaryl
  • Enzyme-linked immunosorbent assay kit for analyzing residual Carbaryl
  • Enzyme-linked immunosorbent assay kit for analyzing residual Carbaryl

Examples

Experimental program
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Effect test

Embodiment 1

[0071] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0072] In the kit, the complex of N-(α-naphthylacetyl)-6-aminocaproic acid and ovalbumin is used as the coating antigen (CBRH-OVA), which is mixed with 0.05mol / L carbonate at pH 9.6 The buffer solution (containing 1g sodium carbonate and 4g sodium bicarbonate, 1L of double distilled water) was diluted to 4 μg / mL, spotted on a 96-well / 40-well microplate, 100 μg / well, and blocked with 5% gelatin.

[0073] The reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl antibody (indirect ELISA), carbaryl standard solution, horseradish peroxidase-labeled goat anti-rabbit antibody (suitable for indirect competitive ELISA) method), horseradish peroxidase-labeled anti-carbaryl rabbit antibody (suitable for direct competition ELISA method), substrate solution and reaction termination solution. The preparation ...

Embodiment 2

[0082] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0083] In the kit, the complex of N-(α-naphthylacetyl)-6-aminocaproic acid and ovalbumin is used as the coating antigen (CBRH-OVA), which is mixed with 0.05mol / L carbonate at pH 9.6 The buffer solution (containing 2g sodium carbonate and 2g sodium bicarbonate, 1L of double distilled water) was diluted to 0.5μg / mL, spotted on a 96-well / 40-well microplate, 100μg / well, and blocked with 5% gelatin.

[0084] The reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl antibody (indirect ELISA), carbaryl standard solution, horseradish peroxidase-labeled goat anti-rabbit antibody (suitable for indirect competitive ELISA) method), horseradish peroxidase-labeled anti-carbaryl rabbit antibody (suitable for direct competition ELISA method), substrate solution and reaction termination solution. The preparation ...

Embodiment 3

[0093] The kit product of the present invention includes a box body, a 96-well / 40-well microtiter plate in the box body and a detection reagent.

[0094] In the kit, the complex of N-(α-naphthylacetyl)-6-aminocaproic acid and ovalbumin is used as the coating antigen (CBRH-OVA), which is mixed with 0.05mol / L carbonate at pH 9.6 The buffer solution (containing 2g sodium carbonate and 2g sodium bicarbonate, 1L of double distilled water) was diluted to 0.5μg / mL, spotted on a 96-well / 40-well microplate, 100μg / well, and blocked with 5% gelatin.

[0095] The reagents in the box include: washing solution (diluent), substrate diluent, anti-carbaryl antibody (indirect ELISA), carbaryl standard solution, horseradish peroxidase-labeled goat anti-rabbit antibody (suitable for indirect competitive ELISA) method), horseradish peroxidase-labeled anti-carbaryl rabbit antibody (suitable for direct competition ELISA method), substrate solution and reaction termination solution. The preparation ...

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Abstract

The invention discloses an enzyme linked immunosorbent assay reagent box applied to carbaryl residual analysis, including box body, enzyme labeled board / test tube and reagent. In each hole of the enzyme labeled board, it uses coating liquor to coat the coating antigen which can peculiarly react with anti-carbaryl antibody, and uses glutin to seal, and the reagent in the box includes washing liquor, substrate diluting liquor, carbaryl standard liquor, anti-carbaryl antibody, enzyme labeled anti-carbaryl antibody, substrate, colour-developing substance and reaction terminating liquor. It can be applied to water, soil, vegetables, and toxic sample, the preprocessing course of the sample is simple and it can synchronously detect batch sample.

Description

(1) Technical field [0001] The invention relates to an enzyme-linked immunosorbent assay kit suitable for analyzing residual carbaryl, the kit is mainly suitable for rapid determination of carbaryl in batches of environmental samples such as water samples, soil, poisoned samples, and food samples such as vegetables. Carbaryl residues. The invention belongs to the field of residual pesticide detection. (2) Background technology [0002] The traditional residue analysis methods of pesticides and their metabolites mainly rely on physical and chemical analysis methods such as gas chromatography (GC), high performance liquid chromatography (HPLC), and mass spectrometry (MS). The chronic and long-term effects of human health and human health are increasingly concerned and worried, and the restrictions on pesticide residues are becoming more and more stringent. New requirements and higher requirements are put forward for the analysis and measurement objects, types, quantities, ran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/535G01N33/558G01N33/573
Inventor 张友军孙家隆王升吉尚佑芬赵玖华杨崇良祁凯徐宝云吴青君路兴波孙红炜
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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