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Interleukin-2 and enterotoxin B fusion gene and its preparation

A technology of fusion gene and interleukin, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc.

Inactive Publication Date: 2005-02-16
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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There is no report on the fusion gene of interleukin-2 and enterotoxin B

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  • Interleukin-2 and enterotoxin B fusion gene and its preparation

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Embodiment 1

[0039] A fusion gene of interleukin-2 and enterotoxin B and its preparation mutant gene, having the sequence shown in SEQ ID NO: 1:

[0040] (1) Information of SEQ ID NO.1 (see sequence listing)

[0041] (a) Sequence features:

[0042] * Length: 1140 bp

[0043] *Type: nucleic acid

[0044] * Chain type: double chain

[0045] *Topology: Linear

[0046] (b) Molecular type: cDNA

[0047] (c) Assumption: No

[0048] (d) Antisense: No

[0049] (e) Original source: interleukin-2 (human interleukin 2), enterotoxin B (Staphylococcal enterotoxin B)

[0050] Material:

[0051] Bacterial strains and E.coli DH5α, JM109 (DE3) are commercially available;

[0052] Plasmid: a plasmid containing the fusion gene, pTE-30a-(+) expression vector, 7ZTS vector (see Chinese patent application 02109681.3) constructed in this example (commercially available 7Z can also be used instead);

[0053] Molecular biology reagents: Taq DNA polymerase, Pfu DNA polymerase, dNTP, hind II...

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Abstract

The invention refers to the technology of generecombination and to be specific it is the fusion gene of the interleukin-2 (IL-2) and enterotoxinB(SEB) and its preparation. It has the sequence table of SEQ ID NO: 1 mid-basic group sequence, based on the natural SEB gene and mustational IL-2 gene, taking advantage of the PCR technology, designing appropriate connecter and connecting the two genes to get fusion gene. The gene can be expressed in the escherichia coli.

Description

technical field [0001] The invention relates to gene recombination technology, specifically a fusion gene of interleukin-2 and enterotoxin B and its preparation. Background technique [0002] IL-2 is a kind of lymphokine that activates T-cells to produce and acts between lymphocytes; human IL-2 is a polypeptide chain consisting of 133 amino acid residues (Rene Devos, Geert Plaetinck, HildeCheroutre, Guus Simons, Wim Degrave, Jan Tavemier, Erik Remaut and WaltrFiers, Molecular cloning of human interleukin 2 cDNA and its expression in E.coli., Nucleic Acids Research, 1983, 11(13): 4307-4323); the molecular weight is 15,000 and IL-2 has been used in recent years Research on tumor therapy has drawn attention to its artificial preparation. [0003] SEB is a single subunit protein, its precursor is composed of 266 amino acids, including a signal peptide composed of 27 N-terminal residues; the precursor is converted into mature SEB after processing by signal peptidase, and its mol...

Claims

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Application Information

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IPC IPC(8): C07H21/04C12N15/26C12P19/34
Inventor 吴文芳杨立泉吕安国时成波
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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