Detecting method of pig metal sulfur protein

A metallothionein and detection method technology, applied in the field of qualitative and quantitative detection of porcine metallothionein, can solve the problems of radioactive contamination, unsatisfactory repeatability, stability and sensitivity, and low sensitivity, and achieve stability and repeatability. Good, avoid radioactive contamination, high sensitivity
CN1619309AInactive Publication Date: 2005-05-25HUNAN AGRICULTURAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUNAN AGRICULTURAL UNIV
Publication Date
2005-05-25
Estimated Expiration
Not applicable · inactive patent
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Abstract

The present invention disclosed a detection method of swine metallothionein, relates to detection method of animal metallothionein, it relates to a qualitative and quantitative detection method of swine metallothionein. Said invention uses electrophoretic pure mouse anti-swine metallothionein IgG as first antibody and uses goat anti-mouse enzyme-linked antibody as second antibody, and adopts solid-phase antigen indirect competitive type enzyme-linked immunosorbent assay method to determine the content of swine metallothionein. Said invention also provides the concrete steps of said method.
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Description

technical field

[0001] The invention relates to a detection method for animal metallothionein, in particular to a qualitative and quantitative detection method for pig metallothionein. Background technique

[0002] Metallothionein (MT) has a strong effect of eliminating free radicals, and can be used alone as a nutritional supplement or in combination with glutathione (GSH), vitamin C (VC), vitamin E (VE), etc., which can effectively inhibit Lipid peroxidation damage, cell protection, lower blood viscosity, improve blood circulation and enhance the body's immunity and other biological functions. Metallothionein can also be used as an antioxidant to prevent oxidative deterioration of food or feed. In addition, metallothionein can combine with many metal ions, participate in the regulation of metal metabolism in the body, balance the homeostasis of metal ions in the body, participate in the operation and excretion of metal ions, and play an important role in mineral nutrition...

Claims

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