Dendrobe protocorm hormoneless cultivation method
A cultivation method and protocorm technology are applied in the field of cultivation of Chinese herbal medicine dendrobium, can solve the problems of few dendrobium resources, long growth cycle of test-tube seedlings, reduced yield and the like, and achieve the effects of shortening growth cycle, fast reproduction speed and short growth cycle
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[0028] Take the wild Dendrobium huoshanense as an example below to illustrate the cultivation method of the present invention.
[0029] Get wild Huoshan Dendrobium seeds (collected from Dabie Mountain, Huoshan County, Anhui Province) and sterilize them with 1% sodium hypochlorite solution for 60 minutes, rinse them with sterile double distilled water for 3 times, inoculate them in a 100ml Erlenmeyer flask filled with 20ml of solid MS medium, and place them at 25 Light at ±2°C, the light condition is 30umol m -2 the s -1 , 16h / day, 4-6cm long sterile test tube seedlings were obtained after induction for 7 months. Solid MS medium consists of the following components: 1900mgL -1 Potassium nitrate, 1650mgL-1 Ammonium nitrate, 170mgL -1 Potassium dihydrogen phosphate, 370mgL -1 Magnesium sulfate heptahydrate, 332.25mgL -1 Calcium chloride, 16.9mgL -1 Manganese sulfate monohydrate, 8.6mgL -1 Zinc sulfate heptahydrate, 6.2mgL -1 Boric acid, 0.83mgL -1 Potassium iodide, 0.25m...
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