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Use of recommbined D-amino acid oxidase

An amino acid, oxidase technology, applied in the field of bioengineering, can solve problems such as low specific activity

Active Publication Date: 2005-10-12
BIORIGHT WORLDWIDE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the specific activity of these two kinds of enzymes catalyzing cephalosporin C is all low (Simonetta, et al., Biochimica et Biophysica Acta, 914:136-142 (1987); U.S.Pat.No.5,453,374; U.S.Pat.No.5,208,155 )

Method used

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  • Use of recommbined D-amino acid oxidase
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  • Use of recommbined D-amino acid oxidase

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Abstract

A recombinative D- amino acid oxidase and its DNA code sequence. Activity to catalysis rhzomorphC of the enzyme is 25% higher than that of its parents at least.

Description

This application is a divisional application of the invention patent application with the application number 200410030842.8 submitted by the applicant Bairui Global Co., Ltd. on April 8, 2004. technical field The invention relates to the technical field of bioengineering, in particular to the preparation and application of a new D-amino acid oxidase. The two high-activity recombinant D-amino acid oxidases provided by the invention can be used to convert cephalosporin C (cephalosporin C) into glutaryl-7-aminocephalosporanic acid (glutaryl-7-aminocephalosporanic acid). Background technique The mother nucleus of semi-synthetic cephalosporins, 7-aminocephalosporanic acid (7-aminocephalosporanic acid), can be obtained by chemically cracking cephalosporin C. However, the chemical method uses a large amount of toxic chemical reagents, which pollutes the environment, and the reaction steps are complicated and the yield is low. In recent years, enzymatic method has become another ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P35/00
Inventor 王骏叶康坚曾伟基吕旭新萧游龙曾实现游明翰
Owner BIORIGHT WORLDWIDE
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