Biodegradable substrate, prosthetic material for tissue regeneration and cultured tissue

A technology of biodegradable and base materials, applied in the field of biodegradable base materials, can solve problems such as difficult manufacturing, low strength, and difficulties

Inactive Publication Date: 2005-12-07
NIPRO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, fabrics and the like having three-dimensional shapes have threads along multiple axes, and it is technically difficult to manufacture collagen threads because they must be woven three-dimensionally.
[0009] In addition, since it is very difficult to manufacture a single thread (collagen thread) with sufficient strength for weaving, and even if it can be produced, its strength is lower than that of synthetic fibers, etc., and it is very difficult to manufacture using the single thread itself.

Method used

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  • Biodegradable substrate, prosthetic material for tissue regeneration and cultured tissue
  • Biodegradable substrate, prosthetic material for tissue regeneration and cultured tissue
  • Biodegradable substrate, prosthetic material for tissue regeneration and cultured tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] [Example 1 Production of Collagen Nonwoven Fabric]

[0079] Pig-derived type I and type III collagen powder (Nippon Ham Co., Ltd., SOFD type, lot number 0102226) was dissolved in distilled water for injection (Otsuka Pharmaceutical Co., Ltd.) to adjust to 7% by weight. Then, this 7% by weight collagen solution was filled into a syringe (manufactured by EFD, disposable tube / piston (Disposable Barrels / Pistons), 55 ml), and the collagen solution was injected into ethanol by air pressure through the needle installed on the syringe. in the slot. At this time, needles of Ultra Dispensing Tips (27G, ID: Φ0.21 mm) manufactured by EFD Corporation were attached to the syringe. After the injected 7% by weight aqueous collagen solution became a dehydrated thread, it was taken out from the ethanol tank. The pulled collagen strands were immersed in a second ethanol tank completely separated from the above-mentioned ethanol tank at room temperature for about 30 seconds to further co...

Embodiment 2

[0080] [Example 2 is processed into a collagen membrane for 2 times]

[0081] Porcine-derived type I and type III mixed collagen powder (Nippon Ham Co., Ltd., SOFD type, lot number 010226) was dissolved in distilled water for injection (Otsuka Pharmaceutical Co., Ltd.) to adjust to 1% by weight. Then, the aqueous collagen solution adjusted to 1% by weight was impregnated into one thermally dehydrated and crosslinked collagen nonwoven fabric obtained in Example 1, formed into a film, and then fully dried naturally at room temperature. Then, thermal dehydration crosslinking reaction was carried out for 12 hours under the conditions of 135° C. and reduced pressure (below 1 torr) in the same vacuum oven as above to obtain a film-like collagen nonwoven fabric.

Embodiment 3

[0082] [Example 3 Production of Collagen Single Thread]

[0083] A 7% by weight aqueous collagen solution was prepared in the same manner as in Example 1. Then, after maintaining the relative humidity of the entire area of ​​the spinning environment described later at 38% or less, the syringe (manufactured by EFD Corporation, Disposable Barrels / Pistons) filled with this 7% by weight aqueous collagen solution was 55 ml), the collagen aqueous solution was injected through the needle mounted on the syringe by applying air pressure. At this time, a needle of Ultra Dispensing Tips (27G, ID: 0.21 mm) manufactured by EFD Corporation was attached to the syringe. The injected 7% by weight collagen aqueous solution was immediately dehydrated and coagulated into a thread shape in an ethanol tank filled with 3 L of 99.5% by volume ethanol (Wako Pure Chemical Industries, Ltd., special grade). The linear collagen pulled out of the ethanol tank was immersed in a second ethanol tank complet...

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Abstract

A biodegradable substrate usable as a prosthetic material (i.e., for tissue regeneration) facilitates the invasion of cells into a substrate and has a thick structure similar to woven fabrics. The biodegradable substrate is obtained by stitching a biodegradable nonwoven fabric with a biodegradable thread. An embodiment of the nonwoven fabric used is a material wherein first and second layers, each having filaments of a thread made of collagen located in parallel, are laminated and adhered to each other so that the alignment direction of the filaments of the thread in the first and second layer are arranged at a certain angle. This nonwoven fabric is stitched with a thread made of collagen. In another embodiment a filmy material having been treated with a biodegradable binder such as collagen or gelatin is piled on the nonwoven fabric and stitched.

Description

technical field [0001] The present invention relates to a biodegradable base material, that is, a medical base material formed of a biodegradable substance represented by collagen. Specifically, it relates to medical treatment for use as various culture base materials such as cell culture base materials for transplantation in regenerative medicine, and as various filling materials and repair materials that promote regeneration induction by filling damaged sites in living bodies. The base material can also be used as a medical base material for various applications such as sustained-release DDS carriers and gene therapy carriers. In addition, the present invention also relates to these uses, in particular, to repair materials and cultured tissues for tissue regeneration. Background technique [0002] In recent years, it has been proposed to use woven or non-woven fabrics made of biodegradable substances such as collagen, gelatin, or hyaluronic acid, or three-dimensional shap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61C13/00A61F2/04A61F2/06A61F2/08A61F2/28A61F2/82A61L27/00A61L27/24A61L27/38A61M29/02B32B5/02B32B5/06B32B5/12B32B5/26C12N5/07C12N5/071D04H3/015D04H3/04
CPCB32B5/06A61L27/24A61L27/3804B32B5/18B32B5/24B32B5/245B32B2307/7163B32B2535/00
Inventor 守永幸弘松田和久神村亮介
Owner NIPRO CORP
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