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Separator, method for manufacturing same, and analysis system

A separation device and separation zone technology, applied in the direction of analyzing materials, instruments, etc., can solve the problem that the resolution cannot always reach a satisfactory level, and achieve the effect of accurate separation

Inactive Publication Date: 2005-12-14
NEC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Resolution isn't always up to par

Method used

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  • Separator, method for manufacturing same, and analysis system
  • Separator, method for manufacturing same, and analysis system
  • Separator, method for manufacturing same, and analysis system

Examples

Experimental program
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Effect test

no. 1 example

[0107] figure 1 An example separation device according to a first embodiment of the invention is shown.

[0108] The separation channel 112 is formed on the substrate 110, and the sample inflow channel 111 and the recovery channel 114 are formed so as to intersect therewith. The sample inflow channel 111, the separation channel 112, and the recovery channel 114 have a fluid reservoir 102a, a fluid reservoir 102b, a fluid reservoir 101a, a fluid reservoir 101b, a fluid reservoir 103a, and a fluid reservoir 103b on their both ends, respectively. Each of these fluid reservoirs has provided thereto electrodes through which an electric field can be applied to both ends of each of the sample inflow channel 111 , the separation channel 112 and the recovery channel 114 .

[0109] The separation channel 112 has a detection unit 113 provided thereto. The external dimensions of the device are reasonably selected according to the purpose of use, and are usually selected within the range...

no. 2 example

[0175] FIG. 21 is a plan view showing in detail the structure of the separation channel 112 according to this embodiment. The general structure of the separating device of the present embodiment and figure 1 The structure of the separation device 100 shown is similar. Also in this embodiment, similarly to the separation channel 112 in the first embodiment, the separation channel 112 has two partition walls 301a and 301b provided therefor. The partition wall 301a and the partition wall 301b respectively formed have a plurality of trapping portions 300 whose opening ratio (opening width / depth) increases in the advancing direction of the flow of the separation channel 112 . In addition, in this embodiment, the partition wall 301a and the partition wall 301b are formed by a series of a plurality of columnar structures 302a, 302b and 302c. The columnar structures 302a, 302b, and 302c are square rods each having a rhombus-shaped base. The columnar structures 302a, 302b, and 302c ...

no. 3 example

[0180] Figure 23 It is a top view showing the structure of the separation channel 112 in detail according to this embodiment. The general structure of the separating device of the present embodiment and figure 1 The structure of the separation device 100 shown is similar. Also in this embodiment, similarly to the separation channel 112 in the first embodiment, the separation channel 112 has two partition walls 301a and 301b provided therefor. In addition, here, the partition wall 301 a and the partition wall 301 b are respectively composed of a plurality of columnar structures 302 , and thus have a plurality of trapping portions 300 . In this embodiment, the partition wall 301a and the partition wall 301b are different from those in the first embodiment and the second embodiment in that they have a plurality of communicating portions 303 formed therein. This allows communication between the channel between the partition wall 301a and the partition wall 301b, the channel be...

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Abstract

A separation device (100) having a separation channel (112), a partition wall (301a) and a partition wall (301b), wherein each of the partition wall (301a) and the partition wall (301b) has a capture portion (300) formed thereon ). Molecules having an acceptable size for the capture moiety (300) located on the partition wall (301a) and the partition wall (301b) are captured by the capture moiety (300) and their travel speed is reduced through the separation channel (112), which enables Molecular size for accurate separation of samples.

Description

technical field [0001] The present invention relates to devices and methods for separating samples, and in particular to devices and methods that can preferably be used to analyze nucleic acid fragments of various sizes including, for example, cells and nucleic acid fragments; organic molecules such as amino acids, peptides and proteins; metal ions, colloids and A device and separation method for fine-scale separation of substances such as latex particles. Background technique [0002] The analysis of biological substances such as cells, nucleic acids and proteins requires prior separation and purification of the samples, or prior separation according to their size and charge. For example, the dideoxy method (Sanger method) is widely used to determine the basic sequence of DNA. During the process of synthesizing complementary DNA by the Sanger method using target single-stranded DNA as a template, Taq polymerase and one of four deoxynucleotides and four dideoxynucleotides a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/08
Inventor 佐野亨马场雅和饭田一浩川浦久雄井口宪幸阪本利司服部涉染谷浩子
Owner NEC CORP