Chitin-sodium alginate packed pylorus helicobacterium protein microballs and their preparation

A technology of Helicobacter pylori and sodium alginate, which is applied in the direction of pharmaceutical formulations, bacterial antigen components, medical preparations of non-active ingredients, etc., can solve the problems of poor affinity of hydrophilic antigens, etc. The effect of release function

Inactive Publication Date: 2006-03-15
ARMY MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) High temperature and other complex preparation environments are often required
(4) Strong hydrophobicity and poor affinity with hydrophilic antigens

Method used

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  • Chitin-sodium alginate packed pylorus helicobacterium protein microballs and their preparation
  • Chitin-sodium alginate packed pylorus helicobacterium protein microballs and their preparation
  • Chitin-sodium alginate packed pylorus helicobacterium protein microballs and their preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of chitosan-sodium alginate-coated Helicobacter pylori protein microspheres

[0042] 1. Take Hp strain M13, culture it in a microaerobic environment (temperature: 37°C; humidity > 95%) in brain heart infusion medium for 24 hours, wash 6 times with 0.05M phosphate buffer saline (PBS, pH7.4), 5000r / Bacteria were collected by centrifugation at 4°C for 10 minutes, and inactivated by adding 5% formaldehyde solution for 24 hours. 200W×30s×10 times of sonication, centrifuged at 15,000g at 4°C for 20min to collect the supernatant, and determined the antigen concentration by Lowry method.

[0043] 2. Preparation of HpWCP-CAMS

[0044] At room temperature, mix the Hp whole bacterial protein prepared in Example 1 with 2% sodium alginate solution, and add vegetable oil, which includes soybean oil, rapeseed oil, peanut oil, sesame oil and the like. The ratio of vegetable oil to sodium alginate emulsion is 2:8, emulsified at 8000r / min for 10min and then added...

Embodiment 2

[0045] Example 2 Detection of chitosan-sodium alginate-coated Helicobacter pylori protein microspheres

[0046] The microspheres prepared above were centrifuged and washed 3 times with distilled water, collected, and vacuum freeze-dried for future inspection.

[0047] 1. Detection of HpWCP-CAMS morphology

[0048] 1) Freeze-drying of MS

[0049] The obtained microsphere suspension was slowly poured into a glass plate, and the liquid level was lower than 1 cm. After pre-freezing in a -40°C refrigerator for 12 hours, place it directly in a pre-cooled vacuum dryer to dry, and slowly raise the temperature to sublimate the water quickly. When the gas pressure indicator shows that no gas is generated, take out the freeze-dried product for inspection.

[0050] 2) Morphological observation of MS before and after drying

[0051] Take MS, lyophilized powder and lyophilized powder reconstituted after washing and centrifuging, and smear a small amount on a glass slide, and observe the ...

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Abstract

A chitosan-sodium alginate wrapped pylorospirobacillus holoprotein microball for developing the slow-release efficient target Hp vaccine with high intestinal target performance is prepared from pylorospirobacillus holoprotein, and the biodegradable chitosan and sodium alginate through emulsifying, solidifying and re-suspending.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to a method for preparing Hp vaccine by wrapping helicobacter pylori (helicobacter pylori, Hp) whole bacterium protein antigen with chitosan and sodium alginate as main materials. Background technique [0002] Helicobacter pylori Hp is the most important pathogenic bacteria that causes human digestive tract diseases such as gastric ulcer, gastritis and even gastric cancer. Since Professor Warren discovered Hp in 1983, people have carried out a lot of research on its vaccine formulation and made some progress. [0003] With the gradual deepening of proteomics research, more and more protein drugs play an important role in the diagnosis, treatment or prevention of various diseases as vaccines. Compared with small-molecule drugs, such drugs have poor stability, are easily degraded by enzymes, and have a short biological half-life, while protein drugs with poor diffusion a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61K9/16A61K47/36A61P1/04
Inventor 邹全明王毅超毛旭虎郭刚任建敏朱永红童文德
Owner ARMY MEDICAL UNIV
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