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Multiple homogeneous tremella polysaccharide, its extraction and medicinal composition with this compound as active components

A technology of tremella polysaccharide and body tremella, applied in the field of several homogeneous tremella polysaccharides

Inactive Publication Date: 2006-05-31
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention obtains two kinds of homogeneous polysaccharides (WTF-A, WTF-B) from the water-soluble part of Tremella spore fermentation powder; from the water-insoluble part of Tremella spore fermentation product, extracts 4 kinds of homogeneous polysaccharides ( ATF-A, ATF-B, ATF-C, ATF-D), are all heteropolysaccharides, especially the lye extract Tremella spore fermentation product, there is no literature report yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0185] (1) Take 150g tremella spore fermentation powder and boil it with 3000ml water for about 5 hours. After cooling to room temperature, the extract was centrifuged, and the residue after extraction was boiled with water, cooled and then centrifuged. The two supernatants were combined and concentrated to slightly viscous with a rotary evaporator. Add three times the amount of ethanol, let stand and filter the precipitate. The precipitate was washed with a little ether and dried in vacuum to obtain 80 g of the product, which was crude polysaccharide.

[0186] (2) Take 10 grams of crude polysaccharide, add 500ml of distilled water, heat to dissolve, after cooling, pour out the supernatant, add 100ml of water to dissolve the insoluble matter. After cooling, centrifuge, take the supernatant, and combine with the previous supernatant to obtain a total of 600ml of supernatant, add 125ml of chloroform and 25ml of n-butanol, and mechanically stir for 20 minutes to produce a milky ...

Embodiment 2

[0189] (1) Water extraction: Take 300g of tremella spore fermented product, add 40 times the amount of water, boil for about 4 hours, and keep the consistency of water during the boiling process. After boiling in water, put it to room temperature, centrifuge the extract, add about 40 times of water to the extracted residue, boil and extract twice in the same way and centrifuge to collect the precipitate. The obtained precipitate was dried in an oven at 50° C., weighed, and the weight was 188.8 g. The precipitate is crude polysaccharide.

[0190] (2) Alkaline extraction: Take 140g of crude polysaccharide, add 0.5mol / L NaOH 1000ml, extract for 6 hours, and centrifuge. The precipitate was extracted two more times in the same way, the supernatants obtained three times were combined, and the precipitate was set aside. The supernatant was concentrated under reduced pressure to about one-third of the original volume, neutralized with 1 mol / L HCl to pH = 7, and the neutralized solut...

Embodiment 3

[0194] Under aseptic conditions, take 18g of tremella polysaccharide in one body, put it in a container, add 1000ml of water for injection, add 50g of mannitol, and 20g of lactose, perform high-pressure sterilization according to the requirements of the injection, add 1.5g of activated carbon, and use microporous filtration Membrane filtration, the filtrate is divided into 0.6ml per bottle, and the yellow loose block is obtained by freeze-drying method, which is sealed.

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PUM

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Abstract

Several homogeneous tremella polysaccharides, its extraction and medicinal composition as the compound as active ingredient are disclosed. The process is carried out by boiling tremella spore fermentative, depositing by alcohol, gradient extracting by different concentration NaOH solution, column chromatography separating, purifying to obtain base-extracted tremella polysaccharide ATF-A, ATF-B, ATF-C and water-extracted tremella polysaccharide WTF-A and WTF-B, and mixing homogeneous tremella polysaccharide with medicinal accessory material to obtain various preparations. It can decrease immunity, delay senility and have antineoplastic and radiation-resistant functions.

Description

technical field [0001] The invention relates to several kinds of polysaccharides of traditional Chinese medicine, more specifically, several homogeneous white fungus polysaccharides obtained by extracting and separating from the white fungus of the traditional Chinese medicine. At the same time, it also discloses its extraction method, and the pharmaceutical composition with the compound as the active ingredient, and the composition is used in the preparation of immune-enhancing, anti-tumor, anti-radiation, increasing white blood cells, anti-virus, anti-aging, anti-AIDS, etc. application in medicine. Background technique [0002] White fungus (Tremella fuciformis Berk abbreviated as TF) claims white fungus again, is a kind of higher fungus, belongs to Tremella fuciformis subclass Tremellaceae. Tremella is recognized as a precious edible fungus and important medicinal material in the world. It has various biological activities, including anti-tumor, anti-virus, anti-inflamma...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P31/12A61P35/00A61P37/00C12P19/04
Inventor 徐文清刘培勋周则卫高小荣李美佳沈秀王月英
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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