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Test paper for lateral flow immunochromatographic semi-quantitative detection of ractopamine

A technology for semi-quantitative detection of ractopamine, applied in the field of β-stimulant residue detection tools, can solve the problems of incomplete detection technology of ractopamine, and achieve the effect of large throughput, fast speed and stable color

Inactive Publication Date: 2006-06-21
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the corresponding ractopamine detection technology in my country is not very complete at present, and it is necessary to develop a simpler, faster and more convenient detection tool

Method used

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  • Test paper for lateral flow immunochromatographic semi-quantitative detection of ractopamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Test paper in the form of 1 detection line + 1 reference line

[0039] The detection line is coated with ractopamine monoclonal antibody or ractopamine detection antigen at a concentration of 0.1-30 μg / mL, with a width of 1 mm; the reference line is coated with an anti-second species animal protein at a concentration of 0.01-30 μg / mL One line of IgG, 1mm wide; the interval between two lines is 2-6mm. When the color of the detection line is the same as that of the reference line, the sample is negative; when the color of the detection line is lighter than the reference line, the sample is positive, and the concentration is greater than Aμg / kg, A is the set detection limit, which can be greater than or equal to A certain concentration of 10 μg / kg. The reference line is always in color, if the reference line does not show color, it indicates that the test paper is invalid.

Embodiment 2

[0040] Example 2 Test paper in the form of 2 detection lines + 1 reference line

[0041] 2 detection lines are coated with 0.01-30 μg / mL ractopamine monoclonal antibody or ractopamine detection antigen, and the concentration of the coating on the second detection line is less than that of the reference line on the first detection line; the reference line Coated with IgG against a second species of animal protein at a concentration of 0.01-30 μg / mL, 1 mm wide; lines spaced 2-6 mm apart. When both detection lines are colored, the sample is negative; when the first detection line of the detection line is colored and the second detection line is not colored, the concentration of ractopamine in the sample is greater than Aμg / kg and less than Bμg / kg kg; when the two detection lines are not colored, the concentration of ractopamine in the sample is greater than Bμg / kg. A and B are the set detection limits, which may be a certain concentration greater than or equal to 10 μg / kg. The ...

Embodiment 3

[0042] Example 3 Test paper in the form of 3 detection lines + 1 reference line

[0043] The 3 detection lines are coated with 0.01-30 μg / mL ractopamine monoclonal antibody or ractopamine detection antigen, and the concentrations of the coatings on the three detection lines are decreased in turn; the reference line is coated with 0.01-30 μg / mL anti- The second species is IgG of animal protein; lines are spaced 2-6 mm apart. When all three detection lines are colored, the sample is negative; when the first and second detection lines are colored and the third detection line is not colored, the concentration of ractopamine in the sample is greater than Aμg / kg, less than Bμg / kg; when the first detection line is colored, and the second and third detection lines are not colored, the concentration of ractopamine in the sample is greater than Bμg / kg and less than Cμg / kg; when the three detection lines are not colored When , the content of ractopamine in the sample bottle is greater t...

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PUM

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Abstract

The invention discloses a semi-quantitative detecting paper of Lac-dopamine effluent immune chromatography, which comprises the following parts on the test paper back: sample liquid absorption part, colloidal gold mark part, detecting reaction part and water-absorbing part, wherein the detecting reaction part contains 1-3 Lac-dopamine antibody as detecting line, which contains 1-3 anti-second animal protein lgG as reference line simultaneously; the amount of combined detecting line and reference line isn't more than one simultaneously; the amount of combined line is 2-4. The invention displays strong specificity to accomplish semi-quantitative detection at 4-40 deg.c temperature for 3 min, which is fit for unit or personnel to detect the Lac-dopamine medicine in the animal source food sample rapidly.

Description

technical field [0001] The invention relates to a detection tool for beta-stimulant residues in animal foods, in particular to a semi-quantitative detection test paper for ractopamine lateral flow immunochromatography and its preparation method and use method. Background technique [0002] Ractopamine is a β-stimulant, commonly known as "clenbuterol", because its residue in animal foods will cause damage to human health, so my country clearly prohibits its use as a nutritional dispensing agent. However, driven by economic interests, illegal abuse of ractopamine still occurs from time to time. [0003] High performance liquid chromatography (HPLC) is currently the main method for the detection of ractopamine residues. However, when HPLC is used, the sample pretreatment is very cumbersome, and the determination method is quite complicated, which requires specially trained personnel to operate, and the detection throughput is very small. Under the current situation of decentr...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N21/78G01N33/52
Inventor 雷红涛孙远明黄晓钰何松伍朝晖吴青王弘谌国莲肖治理潘科
Owner SOUTH CHINA AGRI UNIV
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