Method for detecting and typing 26 human papillomaviruses

A human papillomavirus and detector technology, applied in the biological field, can solve the problems of low sensitivity, high false positive rate, cumbersome operation, etc.

Inactive Publication Date: 2006-08-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

Some of these methods have low sensitivity, some have poor specificity, some are time-consuming, some are prone to cross-infection, have a high false positive rate, and some are cumbersome to operate and cannot be typed

Method used

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Experimental program
Comparison scheme
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Embodiment Construction

[0035] 1. The production method of HPV gene chip is as follows:

[0036]The present invention designs HPV 6, HPV11, HPV16, HPV18, HPV26, HPV31, HPV33, HPV34, HPV35, HPV39, HPV40, HPV42, HPV43, HPV44, HPV45, HPV51, HPV52, HPV53, HPV54, HPV56, HPV58, HPV59, HPV66 , HPV68, HPV73, and HPV82 are type-specific probes for 26 viruses, and were synthesized by Shanghai Yingjun Biotechnology Co., Ltd. Each synthetic probe (Invitrogen, Shanghai, China) had an amino group at its 5' end, followed by a 48 bp type-specific probe. See Table 1 in the Summary of the Invention section for probe sequences.

[0037] Each probe is connected to the corresponding microsphere through a cross-linking reaction.

[0038] The specific method is 5×10 6 Carboxylated microspheres were suspended in 50 μl of 100 mM 2-(N-morpholino)ethanesulfonic acid (MES), pH 4.5 reaction solution, 1 nmol of aminated probe molecules were added, and 25. μg of cross-linking agent N-( After 3-Dimethylaminopropyl)-N-ethylcarbo...

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Abstract

This invention relates to a suspension chip technology for the gene test to human papillomavirus used in testing and typing 26 kinds of papillomaviruses, in which, specific probes of which are crosslinked on 26 kinds of fluorescent microspheres to be reacted with the tested specimens then reacted with the report molecules labeled by the fluorescein to test the type specific nucleic acid of the virus by a fluorescent test device, which can test 26 kinds of ordinary HPV once and increases the test efficiency greatly and overcomes the shortcoming of missing testing the potential infections in the serology and immunity test method to realize early diagnosis to HPA diseases.

Description

technical field [0001] The invention belongs to biotechnology, relates to the fields of molecular biology and biochemistry, and especially relates to a suspension chip technology for human papillomavirus gene detection, which is suitable for clinical detection of human papillomavirus and genotyping, so as to guide the prevention and treatment of cervical cancer . Background technique [0002] As a human pathogenic microorganism, human papillomavirus seriously threatens human health. More than 100 different types of HPV have been identified, and different types of HPV can cause different diseases. HPV infection has been proved by epidemiology and biology to be the primary cause of cervical cancer and its precancerous lesions. Detection and typing of HPV is an effective and reliable means of cervical cancer screening. Due to the difficulty of HPV proliferation in vitro, there is no reliable serotyping method so far. Traditional HPV detection methods mainly detect it through ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 陈智蒋汉梁朱海红
Owner ZHEJIANG UNIV
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