Effective parts of cirald daphne bark, preparation method and application thereof

[0079] Example 1

[0080] 10 kilograms of crushed Zushi hemp medicinal materials are soaked in 15L water for 2 hours, and then filled into percolation buckets, 100L percolation liquid is collected, concentrated, and extracted with petroleum ether three times (1L, 1L and 1L). The water layer is adsorbed on AB-8 resin. The impurities were washed away with 10% ethanol, and then eluted with 60% ethanol. The eluate was concentrated and spray-dried to obtain 510g of the effective part of Zushima. The total coumarin content was measured by spectrophotometry to 78%.

[0081] Example 2

[0082] 10 kg of crushed Zushi Ma medicinal materials were loaded into the reflux device, and extracted with 8L, 8L, and 6L of 20% ethanol for 1.5 hours (3 times in total), combined the extracts, concentrated under reduced pressure and evaporated to remove the ethanol, petroleum ether-ethyl acetate Ester (1∶1) was extracted three times (1L, 1L and 1L), the water layer was adsorbed on HPD-400 resin, washed with water to remove impurities, and then eluted with 70% ethanol, concentrated the eluate, and dried in vacuum to get Zushima effective Part 492g. The total coumarin content is 85% measured by spectrophotometry.

[0083] Example 3

[0084] 10 kg of crushed Zushi Ma medicinal materials were soaked in 10L of 20% ethanol for 12 hours, filtered, and the residue was soaked in 8L of 20% ethanol for 12 hours. The two soaking liquids were combined and the ethanol was recovered under reduced pressure. Petroleum ether-ethyl acetate (2 :1) Extract three times (1L, 1L and 1L), the water layer is directly adsorbed on XAD-4 resin, washed with 20% of impurities, and then eluted with 60% ethanol, the eluate is concentrated, freeze-dried to obtain Zushima The effective part is 560g. The total coumarin content is 90% measured by spectrophotometry.