Method for mass expressing external protein using domestic silk core protein heavy chain promoter

Abstract

The invention relates to a method useing cultivated silkworm fibroin heavy chain promoter to express extraneous sources protein. It can increase extraneous sources protein content to 16% which is greatly higher by using light chain protein and fhx promoter. This can broaden cultivated silkworm application field. And it can be used to easily gain transgene silkworm concoon to get purpose extraneous sources protein by feeding purpose transgene silkworm largely.

Description

Technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for expressing a large number of foreign proteins by using the silkworm fibroin heavy chain promoter. Background technique [0002] The silkworm is an important economic and model organism, which has made great contributions to the development of my country's socialist economy and is making greater contributions to the sustainable development of China's economy. The silkworm genome framework chart led by the National Silkworm Genome Project Research Group of my country was completed first, which not only established the status of our country's silkworm functional genome research in the world, but also provided new opportunities for the development of silk industry and insect science. The main work during the functional genomics period includes the identification and cloning of main functional genes, the elucidation of the molecular mechanisms of important biological trait...

AI Technical Summary

Problems solved by technology

The recently reported production of foreign proteins in the transgenic silk glands of silkworms all use the light chain protein and the fhx promoter. These silkworm expression systems cannot express foreign proteins very effectively because these two components are originally the two components of fibroin. non-essential ingredient

The development of the heavy chain protein promoter, the main component of silkworm fibroin, will help to greatly increase the proportion of exogenous recombinant protein in the transgenic silkworm cocoons, and in silkworm embryos using the transposon piggyBac derived from Lepidoptera In the research report of microinjection transgenic technology, there is no report about the method of using the silkworm fibroin heavy chain promoter to express a large amount of foreign protein