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Calamus effective-part extract and its use

A technology of effective parts and extracts, applied in the field of medicinal use of traditional Chinese medicine, to achieve the effect of simple and reasonable extraction methods

Inactive Publication Date: 2010-05-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no experimental study on the anti-diabetic effect of calamus root

Method used

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  • Calamus effective-part extract and its use
  • Calamus effective-part extract and its use
  • Calamus effective-part extract and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The preparation method of water calamus root extract of the present invention

[0025] After the root of calamus calamus (abbreviated as AC) is crushed, it is extracted with an alcohol solvent lower than 70V / V%, filtered and combined with the filtrate, and the solvent is recovered to obtain a dry paste; the dry paste is suspended in distilled water, and four different polar solvents are used successively , extract with water, n-butanol, ethyl acetate, petroleum ether, recover the solvent in the extract respectively, obtain its water extraction part (Water, be called for short ACW), n-butanol extract (n-Butanol, be called for short ACB), Ethyl Acetate (ACE for short) and Petroleum (ACP for short). The percentages of the obtained weight of each part after vacuum drying corresponding to the weight of the original drug are ACW8.4%, ACB1.6%, ACE1.0%, ACP5.4%.

Embodiment 2

[0026] Example 2 Purification and structural identification of ACE and its isolated components from the root of Acorus calamus

[0027] For further separation components of the ethyl acetate extraction site (ACE), ACE1 is the elution fraction of petroleum ether / ethyl acetate (15:1) of ACE; ACE2 is the elution fraction of petroleum ether / ethyl acetate (9:1) ; ACE3 is the fraction eluted with petroleum ether / ethyl acetate (4:1); ACE4 is the fraction eluted with petroleum ether / ethyl acetate (3:2); ACE5 is the fraction eluted with ethyl acetate.

[0028] The separated components of ACE were trans-dissolved with methanol to obtain a white insoluble matter, which was filtered off, heated to dissolve with chloroform and methanol, applied to Sephadex LH-20, and eluted repeatedly with chloroform / methanol (1:1) to obtain a white monomer, which was mixed with The structure was determined after comparison with the standard.

[0029] After separation, purification and identification, the...

Embodiment 3

[0032] Example 3 In vitro screening and research of the extract parts of Acorus calamus root based on insulin sensitization

[0033] 1. Study on the differentiation-promoting activity of the extract of calamus root on 3T3-L1 cells

[0034] (1) Materials and methods:

[0035] A. 3T3-L1 preadipocyte differentiation

[0036]3T3-L1 cells were seeded in a 24-well plate, and after 2 days of cell fusion, the high-glucose DMEM medium containing 10% FBS, 5 μg / ml INS, 1 μmol / L DEX and 0.5 mmol / L IBMX was replaced, and drugs were added in groups at the same time. AC and its four extraction parts ACW, ACB, ACP, and ACB were set in three concentration groups of 2, 10, and 50 μg / ml. At the same time, a rosiglitazone 1 μmol / L positive control group and a solvent DMSO control group were set up. After 2 days, the DMEM culture solution containing 10% FBS and 5 μg / ml INS was changed and the drugs were added in the same way, and the DMEM culture solution containing only 10% FBS was changed the...

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Abstract

The present invention provides effective Acorus calamus part extract and its medicine composition. The medicine material is Acorus calamus as one kind of Acorus Linn. plant in Araceae. The present invention has simple and reasonable extracting process for extracting effective blood sugar reducing part from Acorus calamus material. The extract has high effect, low toxicity, obvious diabetes treating activity, and may be used in medicine for treating diabetes.

Description

technical field [0001] The invention belongs to the field of medicinal application of traditional Chinese medicines, and relates to an effective fraction of the root extract of Acorus Calamus L. and its application in preparing anti-diabetic drugs. Background technique [0002] Diabetes mellitus is a metabolic disease that causes hyperglycemia due to absolute or relative insufficiency of insulin secretion in the body. Long-term hyperglycemia can cause a series of chronic complications in multiple systems based on vascular disease and neuropathy, which seriously endanger human health. Diabetes has a high incidence in western developed countries; epidemiological studies have shown that in developing countries with rapid economic development and greatly improved people's living standards, the incidence of diabetes is also on the rise. The research and development of anti-diabetic drugs has attracted the attention of the world, and it is a major issue related to people's liveli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7048A61K31/385A61K31/09A61K36/888A61P3/10
Inventor 楼宜嘉朱丹雁吴昊姝郭美媛周长新王志强张翔南马葵芬
Owner ZHEJIANG UNIV
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