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Method and dedicated device for enriching air microorganism

A technology of air microorganisms and enrichment, applied in the direction of supporting/immobilizing microorganisms, measuring/inspecting microorganisms, biochemical equipment and methods, etc., can solve problems such as complex operations, long time required, and low specificity. To achieve the effect of overcoming complex operations, avoiding quantization errors, and overcoming incomplete collection

Inactive Publication Date: 2007-07-11
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, this isolation and culture identification method also has the disadvantages of complex operation, low specificity, and long time required. In addition, many bacterial biochemical characteristics, antibiotic-sensitive phenotypic characteristics are unstable, and are easily affected by gene regulation, plasmid acquisition, etc. Therefore, it is easy to cause false negative results and lead to missed or misdiagnosed

Method used

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  • Method and dedicated device for enriching air microorganism
  • Method and dedicated device for enriching air microorganism
  • Method and dedicated device for enriching air microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] The purpose of the experiment: to monitor the presence of SARS virus in the ambient air of places with high crowd flow

[0117] Sources of experimental samples: Air samples in the waiting room of Shenzhen Railway Station and the waiting room of Shenzhen Futian Long-distance Bus Station

[0118] Experimental steps:

[0119] (1) Preparation before collection

[0120] (1) Open the collecting net tank before the filter membrane in the filter collection assembly in the collection device, put the filter membrane on the mesh liner behind the filter membrane, cover the front collecting net tank of the filter membrane, and autoclave together with the front and rear sealing covers deal with.

[0121] (2) After stopping the disinfection, cover the filter collection assembly with the front and rear sealing caps under aseptic conditions.

[0122] (2) Sample collection: At 10:00 am and 3:00 pm, air samples were collected twice in the waiting room of Shenzhen Railway Station and Sh...

Embodiment 2

[0137] Purpose of the experiment: To monitor the presence of Mycobacterium tuberculosis in the ambient air of a hospital

[0138] Source of experimental samples: air samples in the outpatient hall of Shenzhen Nanshan District People's Hospital

[0139] Experimental steps:

[0140] (1) Preparation before collection: the same as in Example 1 above.

[0141] (2) Sample collection: At 10:00 am and 3:00 pm, air samples were collected twice at different locations in the outpatient hall of the People's Hospital of Nanshan District, Shenzhen, and collected for three consecutive days. There are 12 samples in total. All the other steps are the same as in Example 1.

[0142] (3) DNA extraction of samples:

[0143](1) Remove the rear sealing cover of the filter collection assembly under aseptic conditions, open the front sealing cover, put the filter collection assembly into the buffer solution, vortex and oscillate for cleaning; rinse with sterile water twice; collect all cleaning so...

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Abstract

The invention discloses an enriched air microbe biological device and method, which comprises the following parts: case, which possesses outlet on one end and closing end with porous network; filtering collecting component, which is connected on the outlet end of case detachably; fan component, which consists of wheel and motor in connection with wheel in the case, wherein the blade end of wheel opposes the filtering collecting component. The invention enriches all microbes to suck under certain time and certain bulk directly, which purifies and extracts nucleic acid of microbe effectively.

Description

technical field [0001] The invention relates to a device for enriching airborne microorganisms and a method for enriching airborne microorganisms with the device. Background technique [0002] With the improvement of material living standards, people's requirements for air quality are also getting higher and higher. Air pollution, especially the harm of pathogenic microorganisms in the air to human health is being paid more and more attention. Many important human infectious diseases can be transmitted through the air. Studies have shown that the prevalence of hospital-acquired infections is 3% to 20%, and respiratory infections caused by airborne microorganisms account for about 15% to 20%. For example, the SARS virus that broke out in my country and its surrounding areas in 2003 was mainly spread in the air in the form of aerosol, which is a typical respiratory-air-respiratory disease; another example It is the H5N1 avian flu disease that occurred recently. In addition to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12Q1/00C12Q1/68
Inventor 顾大勇李长春吕志平徐云庆吉雁鸿张雅鸥杨景涛周元国鲁卫平禹华伟梁冰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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