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Method and dedicated device for enriching air microorganism

An airborne microorganism and enrichment technology, which is applied in the methods of supporting/immobilizing microorganisms, the determination/inspection of microorganisms, biochemical equipment and methods, etc. Achieve the effect of overcoming incomplete collection, avoiding quantification errors, and overcoming complex operations

Inactive Publication Date: 2009-07-29
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, this isolation and culture identification method also has the disadvantages of complex operation, low specificity, and long time required. In addition, many bacterial biochemical characteristics, antibiotic-sensitive phenotypic characteristics are unstable, and are easily affected by gene regulation, plasmid acquisition, etc. Therefore, it is easy to cause false negative results and lead to missed or misdiagnosed

Method used

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  • Method and dedicated device for enriching air microorganism
  • Method and dedicated device for enriching air microorganism
  • Method and dedicated device for enriching air microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0116] The purpose of the experiment: to monitor the presence of SARS virus in the ambient air of places with high crowd flow

[0117] Sources of experimental samples: Air samples in the waiting room of Shenzhen Railway Station and the waiting room of Shenzhen Futian Long-distance Bus Station

[0118] Experimental steps:

[0119] (1) Preparation before collection

[0120] (1) Open the collecting net tank before the filter membrane in the filter collection assembly in the collection device, put the filter membrane on the mesh liner behind the filter membrane, cover the front collecting net tank of the filter membrane, and autoclave together with the front and rear sealing covers deal with.

[0121] (2) After stopping the disinfection, cover the filter collection assembly with the front and rear sealing caps under aseptic conditions.

[0122] (2) Sample collection: At 10:00 am and 3:00 pm, air samples were collected twice in the waiting room of Shenzhen Railway Station and Sh...

Embodiment 2

[0137] Purpose of the experiment: To monitor the presence of Mycobacterium tuberculosis in the ambient air of a hospital

[0138] Source of experimental samples: air samples in the outpatient hall of Shenzhen Nanshan District People's Hospital

[0139] Experimental steps:

[0140] (1) Preparation before collection: the same as in Example 1 above.

[0141] (2) Sample collection: At 10:00 am and 3:00 pm, air samples were collected twice at different locations in the outpatient hall of the People's Hospital of Nanshan District, Shenzhen, and collected for three consecutive days. There are 12 samples in total. All the other steps are the same as in Example 1.

[0142] (3) DNA extraction of samples:

[0143] (1) Remove the rear sealing cover of the filter collection assembly under aseptic conditions, open the front sealing cover, put the filter collection assembly into the buffer solution, vortex and oscillate for cleaning; rinse with sterile water twice; collect all cleaning sol...

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Abstract

The invention discloses a device and a method for enriching air microorganisms. The device for enriching airborne microorganisms provided by the present invention comprises: a shell, one end of which is open, the other end is closed, and the closed end has a porous grid; a filter collection assembly, which is detachably connected to The open end of the housing; the fan assembly, which includes a leaf wheel and a motor connected to the leaf wheel, is located inside the housing; the blade end of the leaf wheel faces the filter collection assembly. The invention adopts the principle of direct inhalation and filtration to filter and enrich all microorganisms in a certain volume of air within a certain period of time, then extract and purify nucleic acids from the enriched microorganisms, and then combine the corresponding molecular microbiology detection method Conduct detection and identification.

Description

technical field [0001] The invention relates to a device for enriching airborne microorganisms and a method for enriching airborne microorganisms with the device. Background technique [0002] With the improvement of material living standards, people's requirements for air quality are also getting higher and higher. Air pollution, especially the harm of pathogenic microorganisms in the air to human health is being paid more and more attention. Many important human infectious diseases can be transmitted through the air. Studies have shown that the prevalence of hospital-acquired infections is 3% to 20%, and respiratory infections caused by airborne microorganisms account for about 15% to 20%. For example, the SARS virus that broke out in my country and its surrounding areas in 2003 was mainly spread in the air in the form of aerosol, which is a typical respiratory-air-respiratory disease; another example It is the H5N1 avian flu disease that occurred recently. In addition to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/12C12Q1/00C12Q1/68
Inventor 顾大勇李长春吕志平徐云庆吉雁鸿张雅鸥杨景涛周元国鲁卫平禹华伟梁冰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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