EB matrix production from fetal tissues and its use for tissue repair

Inactive Publication Date: 2002-12-05
TEI BIOSCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, aldehydic processing effectively destroys any biological activity, such as cell binding sites, associated with the original tissue and greatly reduces or eliminates the ability of cells to attach to it.
Collagen-based devices that are animal-derived and fixed with glutaraldehyde or a similar agent can not be rem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

Preparation of an EBM Blood Vessel

[0024] The preferred protocol to prepare EBM derived from a blood vessel is as follows. All of the following steps are performed in a laminar-flow hood using aseptic techniques and sterile solutions.

[0025] A blood vessel is cut out from the body of an animal. Flesh is removed from the blood vessel, and the artery is placed on a 4 mm mandrill. Flesh can be removed from the blood vessel using dissection tools, if done by hand; serrated tipped forceps are used to lift the flesh, and curved scissors are used to remove the flesh. Defleshing can also be performed by a defleshing machine. If carried out manually, the process should continue as follows.

[0026] The blood vessel is placed in 20% bleach for 30.+-.2 minutes on a rocking platform on ice. The temperature of the bleach is 4.+-.2.degree. C. The volume of the bleach is 500.+-.50 mls in a 1.0 liter square, wide-mouth bottle with a screw cap. The blood vessel is dipped into 1.0 liters of Milli-Q.TM. wa...

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Abstract

A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.

Description

[0001] This application is a continuation-in-part application of U.S. application Ser. No. 09 / 871,518, filed May 31, 2001, the entire contents of which are herein incorporated by reference.[0002] 1. Field of the Invention[0003] This invention relates to the field of tissue engineering, and in particular to animal-derived, bioremodelable, biopolymer scaffold materials used to repair animal tissue. The term "bioremodelable" or "bioremodelability" refers to a material that lends itself to the breakdown by cells that occupy it and use it as a template for creating a replacement made up mainly of newly synthesized components secreted by the cells.[0004] Rebuilding the human body is a significant industry. Human tissue banks and synthetic polymers do not meet the need for repair or replacement of body parts. High on the list of alternative sources of material used to meet this need are animal tissues prepared in new ways that reduce their immunogenicity and maximize their usefulness and e...

Claims

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Application Information

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IPC IPC(8): A61L27/00A61K35/12A61K35/44A61L27/36C12N5/08
CPCA61L27/3604A61L27/3625A61L27/3683A61L27/3687A61K38/1875A61L27/3834A61L2430/40A61K35/44A61L27/3691A61P17/02A61P19/04C12N5/0602C12N2533/90C12N2537/00C12N2537/10
Inventor DAI, JIANWUBELL, EUGENERUSSAKOVSKY, VLADIMIR
Owner TEI BIOSCI
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