Compositions and methods for inhibiting 5-alpha reductase
a technology of reductase and reductase, which is applied in the field of compounds and methods for treating disease, can solve the problems that the normal pattern of female hair growth is unacceptable to many women, and achieve the effects of modulating androgenic activity, reducing body weight, and inhibiting the formation and availability of active androgens
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(1) Fractionation of Serenoa Repens Berries
[0059] In order to establish optimal conditions for preparing an extract from Serenoa repens (Saw Palmetto) berries, Super Critical Fluid (SCF) fractionation was carried out on the apparatus, shown as FIG. 2. This apparatus comprises a dual pump system, utilizing one syringe pump for neat supercritical near-critical or critical fluid and a second syringe pump for an alcohol co-solvent or modifier. The pumps are independently controllable, allowing easy adjustment of fluid composition.
[0060] To prepare a sample, the Serenoa repens biomass was ground into a fine powder (around 100 mesh). Three grams of the dried powder was transferred to a 10 mL extraction cartridge, after which the cartridge was filled with glass wool or beads to reduce the dead volume. After loading a cartridge on its holder, a sequential extraction / fractionation procedure was commenced. The system was then brought to 3,000 psig and 40° C., and extracted for 30 minutes w...
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