Viruses encoding mutant membrane protein

a virus and membrane protein technology, applied in the field of viruses encoding mutant membrane proteins, can solve the problems of obviating difficulties, unable to interrupt the electrophysiological measurement of nb protein based on the lipid bilayer system, and unable to meet the needs of patients, etc., to achieve the effect of altering the reading fram

Inactive Publication Date: 2010-09-30
WISCONSIN ALUMNI RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the electrophysiological measurements of NB protein based on the lipid bilayer system are difficult to interrupt.
Thus, the available evidence challenges the notion that the NB protein has ion channel activity.
Live virus vaccines activate all phases of the immune system and stimulate an immune response to each of the protective antigens, which obviates difficulties in the selective destruction of protective antigens that may occur during preparation of inactivated vaccines.
However, the mutations in attenuated virus are often ill-defined and those mutations appear to be in the viral antigen genes.

Method used

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  • Viruses encoding mutant membrane protein
  • Viruses encoding mutant membrane protein
  • Viruses encoding mutant membrane protein

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example 1

Materials and Methods

[0076]Cells and viruses. 293T human embryonic kidney cells and Madin-Darby canine kidney cells (MDCK) were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal calf serum and in modified Eagle's medium (MEM) containing 5% newborn calf serum, respectively. All cells were maintained at 37° C. in 5% CO2. Influenza viruses A / WSN / 33 (H1N1) and A / PR / 8 / 34 (H1N1) were propagated in 10-day-old eggs.

[0077]Construction of plasmids. To generate RNA polymerase I constructs, cloned cDNAs derived from A / WSN / 33 or A / PR / 8 / 34 viral RNA were introduced between the promoter and terminator sequences of RNA polymerase I. Briefly, the cloned cDNAs were amplified by PCR with primers containing BsmBI sites, digested with BsmBI, and cloned into the BsmBI sites of the pHH21 vector which contains the human RNA polymerase I promoter and the mouse RNA polymerase I terminator, separated by BsmBI sites (FIG. 2). The PB2, PB1, PA, HA, NP, NA, M, and NS genes of the ...

example 2

Materials and Methods

[0092]Cells, viruses, and antibodies. 293T human embryonic kidney cells and Madin-Darby canine kidney (MDCK) cells were maintained in DMEM supplemented with 10% fetal calf serum and in MEM containing 5% newborn calf serum, respectively. The 293T cell line is a derivative of the 293 line, into which the gene for the simian virus 40 T antigen was inserted (DuBridge et al., 1987). All cells were maintained at 37° C. in 5% CO2. B / Lee / 40 and its mutant viruses were propagated in 10-day-old embryonated chicken eggs. The viruses were purified from allantoic fluid by differential centrifugation and sedimentation through a 10-50% sucrose gradient. An anti-NB rabbit serum was generated against synthesized peptide NKRDDISTPRAGVD (SEQ ID NO:9; amino acid residues 70-83 of NB protein) coupled to keyhole limpet hemocyanin.

[0093]Construction of plasmids. The cDNAs of B / Lee / 40 viruses were synthesized by reverse transcription of viral RNA with an oligonucleotide complementary t...

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Abstract

A method to prepare viruses with a mutant membrane protein gene, and viruses obtained by the method, are provided.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The present application is a continuation of U.S. patent application Ser. No. 10 / 827,995, filed Apr. 20, 2004, which claims the benefit of the filing date of U.S. application Ser. No. 60 / 464,776, filed Apr. 23, 2003, and U.S. application Ser. No. 60 / 465,328, filed Apr. 24, 2003, the disclosures of which are incorporated by reference herein.STATEMENT OF GOVERNMENT RIGHTS[0002]This invention was made with a grant from the Government of the United States of America (grant AI-47446 from the National Institutes of Health). The Government may have certain rights in the invention.BACKGROUND OF THE INVENTION[0003]Cell membranes consist of a double layer of lipid molecules in which various proteins are embedded. Because of its hydrophobic interior, the lipid bilayer of cell membranes serves as a barrier to the passage of most polar molecules and therefore is crucial to cell viability. To facilitate the transport of small water-soluble molecules in...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/145A61P37/04C12N7/01A61K39/00A61K48/00C07K14/11C12N7/00C12N7/04C12N15/44
CPCA61K39/00A61K48/00A61K2039/5254A61K2039/5256C07K14/005C12N7/00C12N2760/16322C12N2760/16062C12N2760/16121C12N2760/16122C12N2760/16123C12N2760/16134C12N2760/16222C12N2760/16043A61P31/12A61P31/16A61P37/04C12N7/04C07K14/11
Inventor KAWAOKA, YOSHIHIRO
Owner WISCONSIN ALUMNI RES FOUND
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