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Methods to prevent cytotoxicity using n-acetyl-cysteine

a technology of n-acetylcysteine and cytotoxicity, which is applied in the field of methods to prevent cytotoxicity using n-acetylcysteine, can solve the problems of significant toxicity to the cells lining the tooth, and achieve the effect of preventing toxicity

Inactive Publication Date: 2011-07-07
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides an inhibitor of the toxicity of conventional restorative materials used in dentistry. The invention demonstrates the effectiveness of N-acetyl-cysteine (NAC) in reducing the adverse effects of resin monomers, such as HEMA, on cells lining the tooth. The restorative material can cause toxicity, leading to various diseases such as allergies and tooth loss. The invention provides a solution by including NAC in the restorative material or as a separate material from the restorative material. The effective amount of NAC can be about 10 mM to about -30 mM of the restorative material or about 20 mM. The invention prevents cytotoxicity, allergies, tooth loss, and other effects caused by the restorative material.

Problems solved by technology

When teeth from humans are restored with conventional restorative materials, the restorative materials can cause significant toxicity to the cells lining the tooth, often causing pathologies ranging from simple tooth sensitivity to food and fluid intake, the need for a root canal, and to more serious disease states, including allergies to the restorative material.

Method used

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  • Methods to prevent cytotoxicity using n-acetyl-cysteine
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  • Methods to prevent cytotoxicity using n-acetyl-cysteine

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Embodiment Construction

[0065]Exposure to hydroxyethyl methacrylate (HEMA) significantly induces apoptotic cell death in a dose-dependent manner (Paranjpe et al. 84(2), J. Dent. Res. 2005). The present studies demonstrate, among other things, that this cell death can be inhibited by N-acetyl cysteine (NAC), providing a mechanism to ameliorate some of the negative effects of HEMA in patient treatment.

[0066]Unless otherwise provided, the following generalized methods were used in the experiments described below. Cell lines were stimulated with concentrations of HEMA ranging from 0.082M to 0.000182M. Stimulations occurred for time periods ranging from about 4 to about 24 hours. NAC stock solutions (1 M) were made in N[2-hydroxyethyl]piperazine-N′-[2-ethanesulfonic acid] (HEPES) and 10M NaOH immediately before use and were diluted to a final concentration of 20 mM in culture medium. NAC was added either simultaneously with HEMA or added about 1 to about 2 hours before the addition of HEMA to the cells. HEMA, N...

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Abstract

Compositions and methods to inhibit adverse physiological effects caused by resin-based and resin-containing materials are disclosed. More specifically, the use of N-acetyl-cysteine (NAC) to inhibit hydroxyethyl methacrylate (HEMA)-mediated cell death and cytotoxicity.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]The present application claims the benefit under 35 USC 371 to PCT / US2007 / 060025 filed on Jan. 2, 2007, 35 U.S.C. §119(e) to U.S. Provisional Patent Application Nos. 60 / 755,947, filed Jan. 3, 2006 and 60 / 805,829, filed Jun. 26, 2006, the disclosures of which are incorporated herein in their entirety.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT[0002]This invention was made with United States Government support of Grant No. DE10331 awarded by the National Institutes of Health. The United States Government has certain rights in this invention.FIELD OF THE INVENTION[0003]The present invention relates to resin-based and resin-containing materials and bleaching agents commonly used in dental and other practices. More specifically, the present invention relates to compositions and methods to inhibit adverse physiological effects caused by these materials. More specifically, the present invention relates to the use of N-acetyl-...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K8/44C07C321/14A61Q11/02A61K31/198A61P37/08A61P43/00A61P17/12A61P21/00
CPCA61K6/0017A61K6/0023A61K6/0067A61K6/083A61K6/0835C08L33/10A61K6/20A61K6/30A61K6/69A61K6/887A61K6/889A61P17/12A61P21/00A61P37/08A61P43/00
Inventor JEWETT, ANAHIDPARANJPE, AVINA
Owner RGT UNIV OF CALIFORNIA