Lactobacillus sp. strain having ability to inhibit proliferation of virginal pathogenic microorganisms

a technology of lactobacillus and sneathia spp, which is applied in the field of lactobacillus sp. strain, can solve the problems of increasing the recurrence rate, decreasing the abundance of sneathia spp, and accompanied by various side effects of vaginal antibiotic use, so as to improve the survival rate, prevent and/or treat the effect of sneathia spp growth

Inactive Publication Date: 2018-05-03
KO BIOLABS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0044]The present invention relates to a novel Lactobacillus sp. strain having an ability to inhibit the growth of pathogenic vaginal microorganisms, and a pharmaceutical composition, a health functional food, and a cleansing product containing the same as an active ingredient. Therefore, the present invention exhibits an activity to inhibit the growth of Sneathia spp. pathogens associated with the human papillomavirus (HPV) infection and the occurrence of bacterial vaginitis, and an inhibitory effect on the growth of Gardnerella vaginalis which is a vaginitis pathogen, and Candida albicans which is a causative bacteria of Candidal vaginitis, and thus can be used for the improvement, prevention and / or treatment of female vaginitis.

Problems solved by technology

Conventional treatment of vaginitis using antibiotics is accompanied by various side effects, such as an increase in recurrence rate, a decrease in abundance of Lactobacillus spp., the generation of inflammation caused by other antibiotic resistant strains besides Gardnerella sp. and Candida sp.

Method used

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  • Lactobacillus sp. strain having ability to inhibit proliferation of virginal pathogenic microorganisms
  • Lactobacillus sp. strain having ability to inhibit proliferation of virginal pathogenic microorganisms
  • Lactobacillus sp. strain having ability to inhibit proliferation of virginal pathogenic microorganisms

Examples

Experimental program
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Effect test

example 1

and Identification of Strains

[0051]About 190 different Lactobacillus strains were isolated from the vaginal microflora of healthy women. Specifically, the samples for the isolation of vaginal microflora were obtained from nine subjects, including three pairs of identical twins and their mothers who participated in the Korean Twin-Family Cohort Study, and were supplied from Samsung Hospital in the form of mid-vaginal swab samples (IRB No. 144-2011-07-11).

[0052]The swab samples were transferred to the present laboratory in the form of being stored in a refrigerator within 4 hours after collection in a modified Liquid Amies solution and immediately used for the isolation of strains. The samples were sequentially diluted from 10−1 times to 10−8 times and spread on three different media of Chocolate agar, Rogosa agar, and Columbia agar, and cultured for 48 hours under anaerobic conditions.

[0053]After the culture, purely isolated colonies were randomly selected and subjected to shake cult...

example 2

bition Assay

[0057]Each of Lactobacillus strains isolated in Example 1 was used for the assay by culturing at 37° C. for 20 hours using MRS broth (Difco, USA). The strains of Sneathia spp. and Gardnerella vaginalis were inoculated into NYCIII broth and then anaerobically cultured at 37° C. for 48 hours or 24 hours, respectively, and used in the experiment.

[0058]As for the Sneathia spp. strains used in the experiment, two kinds of strains of Sneathia spp., which was isolated in Virginia Commonwealth University School of Medicine and has been reported in the paper, and Sneathia sanguinegens that the present inventors isolated from Korean women were used. As the Gardnerella vaginalis strain used in the experiment, KCTC 5096 strain furnished from the Korean Collection for Type Cultures (KCTC) was used.

[0059]After dispensing and solidifying 15 ml of MRS agar medium on a plate, 7 ml of NYCIII soft agar medium (0.75% agar) inoculated with the strains of Sneathia spp. and Gardnerella vaginal...

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Abstract

The present invention relates to a novel Lactobacillus sp. isolation strain having an activity to inhibit the growth of vaginitis pathogens, and a pharmaceutical composition, a health functional food, and a cleansing product, comprising the strain as an active ingredient. Therefore, the present invention exhibits an effect of inhibiting the growth of Sneathia spp. pathogens associated with the infection with human papillomavirus (HPV) and the incidence of bacterial vaginitis, Gardnerella vaginalis as a vaginitis pathogen, and Candida albicans as a causative bacteria of Candidal vaginitis, and an effect of recovering and maintaining the vaginal microflora, and thus can be used for the prevention and treatment of female vaginitis.

Description

TECHNICAL FIELD[0001]The present invention activity relates to a Lactobacillus sp. strain having an ability to inhibit the growth of pathogenic vaginal microorganisms, a growth inhibitor of pathogenic vaginal microorganisms including the same as an active ingredient, and a product for improving, preventing or treating vaginitis including the inhibitor. More specifically, the present invention relates to a novel Lactobacillus sp. strain having an inhibitory effect on the growth of pathogenic vaginal microorganisms wherein the pathogenic vaginal microorganisms include fungi, bacteria and viruses, and specific examples thereof include Candidia spp. strains, Gardnerella spp. strains, or Sneathia spp. strains, or the like, and a pharmaceutical composition, a health functional food or a cleansing product including the same as an active ingredient.BACKGROUND ART[0002]Globally, three-quarters of the female population is experiencing an infection of inflammatory diseases of the female genita...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K35/747C12R1/225A61P31/04A61P31/10A61P31/20A61P15/02A23L33/00A23L33/135A23L2/52A23C9/123
CPCA61K35/747C12R1/225A61P31/04A61P31/10A61P31/20A61P15/02A23L33/40A23L33/135A23L2/52A23C9/1234A23V2002/00A23Y2220/00C12R2001/225C12N1/205A23V2400/149A23V2400/11A23V2400/145A23V2400/143C11D9/04C12N1/20A23V2200/00
Inventor KO, GWANG PYOYOU, HYUN JUKWON, BO MI
Owner KO BIOLABS INC
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