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Production of alpha-olefins

a technology of alpha-olefin and alpha-olefin, which is applied in the direction of lyases, polypeptides with his-tags, lubricant compositions, etc., can solve the problems of mixing that may not be desired, and achieve specific decarboxylase activity, facilitate the identification of similar enzymes, and specific decarboxylase activity

Inactive Publication Date: 2018-06-21
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is based on the discovery that certain genomic sequences of Alicyclobacillus acidocaldarius and Staphylococcus massiliensis encode enzymes with free fatty acid decarboxylase activity. These enzymes produce medium-chain α-olefins, such as C7 to C11, from medium-chain free fatty acids. The invention provides examples of these enzymes and the identification of similar enzymes from other organisms.

Problems solved by technology

Such mixture may not be desired as feedstock for further chemical conversion such as the production of poly-α-olefins of interest.

Method used

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Examples

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example 3

n of α-Olefins and Hydroxy Fatty Acids by Sm46-Del29, Bs168 and Aa162

[0170]Recombinantly expressed and purified Bs168 and Aa162 from Example 1 and Sm46-de129 of Example 2 were reacted with C14 fatty acid and the products of decarboxylation (C13 α-olefin) and hydroxylation (α-OH—O14 fatty acid and β-OH—C14 fatty acid) reactions were analyzed.

[0171]All enzymes tested were able to decarboxylate myristic acid (C14), but also catalyzed α- and β-hydroxylation of myristic acid as side reactions. Fatty acid decarboxylation was the dominant reaction for all tested enzymes, but Sm46-del29 formed less hydroxy fatty acids, indicating that these enzymes have specific decarboxylase activity.

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Abstract

The present invention relates to the biosynthesis of α-olefins. In particular, the invention provides methods for the production of medium-chain α-olefins, more particularly C11 α-olefins, using a polypeptide with decarboxylase activity on free fatty acids with 8 to 14 carbons, in particular on C8-C12 free fatty acids, more particularly on C12 free fatty acids, or a genetically engineered host cell expressing or overexpressing said polypeptide.

Description

TECHNICAL FIELD[0001]The application generally relates to biosynthetic processes. In particular, the application relates to the biosynthesis of α-olefins.BACKGROUND[0002]Unsaturated hydrocarbons such as α-olefins are an industrially important group of molecules which can serve as precursors for lubricants and surfactants in addition to being used in fuels, or as feedstock for further chemical conversion to e.g. fuels, polymers, plastics, textiles, solvents, adhesives, etc.[0003]α-Olefins, more particularly linear α-olefins, have traditionally been produced from petroleum sources through oligomerization of ethylene but it would be advantageous to find alternative methods for producing such α-olefins, notably from renewable resources. Moreover, the chemical synthesis results in a mixture of linear α-olefins which exhibit different carbon chain length. Such mixture may not be desired as feedstock for further chemical conversion such as the production of poly-α-olefins of interest.[0004...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C10M105/04C12P5/02C12N9/88
CPCC10M105/04C12P5/026C12N9/88C10N2240/10C10M2203/024C10N2270/00C07K2319/21C10M2205/0285C10N2040/25C10N2070/00
Inventor FOURAGE, LAURENTLAEUFFER, FREDERICSTRUB, HENRIWANG, YUNXU, JIANHU, HUIFANGLI, SHENGYING
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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