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Microorganism having acyltransferase activity and uses thereof

a technology of acyltransferase activity and microorganisms, which is applied in the direction of acyltransferases, enzymology, transferases, etc., can solve the problem of actual production of acetylmethionine, and achieve the effect of enhancing acyltransferase activity and efficiently using production

Inactive Publication Date: 2019-08-29
CJ CHEILJEDANG CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present patent provides a microorganism that efficiently produces N-acetyl-L-methionine by acetylating L-methionine using an acyltransferase with enhanced activity. The microorganism has a polypeptide with a high degree of homology to certain amino acid sequences. The acyltransferase can be derived from genera such as Bacillus, Pseudomonas, Enterobacter, and Corynebacterium. The polypeptide may have an amino acid sequence of SEQ ID NO: 1 to 6 or a polypeptide with a similar structure that performs the same function. The patent also mentions the use of a specific alignment program to determine the degree of identity between sequences.

Problems solved by technology

Further, it has not been found that N-acetylmethionine is actually produced by the transformant transformed by YncA.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

of Novel Enzyme for Producing N-acetylmethionine

example 1-1

f Microorganism for Producing N-acetyl-L-methionine

[0056]As microorganisms having an ability of producing N-acetyl-L-methionine, N-acetylmethionine spots, which are products, were confirmed from six kinds of randomly selected microorganism culture solutions (Pseudomonas putida, Bacillus subtilis, Enterobacter sp. 638, Pseudovibrio sp. FO-BEG1, Yarrowia lipolytica PO1f (ATCC MYA-2613™), and Corynebacterium glutamicum ATCC 13032).

[0057]Specifically, Pseudomonas putida, Bacillus subtilis, Enterobacter sp. 638, or Yarrowia lipolytica PO1f was inoculated in a 14 mL disposable culture tube containing 3 mL of a liquid YPD medium (1% yeast extract, 2% bacto-peptone, 2% glucose), and was shake-cultured for 24 hours under conditions of 30° C. and 200 rpm to obtain a seed culture solution. 1 mL of the seed culture solution was inoculated in a 250 mL corner-baffle flask filled with 24 mL of the liquid YPD medium containing 0.5% (5 g / L) of methionine, and was shake-cultured for 24 hours under co...

example 1-2

n of Novel Acyltransferase Sequence

[0064]Methionine acyltransferase was selected from the six kinds of microorganisms selected in Example 1-1.

[0065]Heterogeneous homology searches for the selected proteins were performed by restricting the database to species of the microorganism based on an amino acid sequence with YncA known as a conventional methionine acyltransferase. As a result, all of the six kinds of searched enzymes were found to have low homology with the YncA amino acid sequence, and the homology of the Enterobacter sp. 638-derived acyltransferase sequence, which had the highest homology, was 82%. Homologies of the respective yeasts with YncA are given in Table 1.

[0066]From the above results, it is inferred that the above kinds of polypeptides are polypeptides having novel acyltransferase activity lower than the homology of a conventional acyltransferase, and the microorganisms including these polypeptides are also microorganisms having novel acyltransferase activity whic...

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Abstract

The present disclosure relates to a polypeptide having an acyltransferase activity or a microorganism including the same; a composition for preparing N-acetyl-L-methionine, the composition including the polypeptide or microorganism; and a method of preparing N-acetyl-L-methionine using the polypeptide or microorganism. Further, the present disclosure relates to a polynucleotide encoding the polypeptide and an expression vector including the polynucleotide. Since the microorganism including a novel acyltransferase according to the present disclosure has enhanced acyltransferase activity, this microorganism can be efficiently used for producing N-acetyl-L-methionine by acetylating L-methionine.

Description

TECHNICAL FIELD[0001]The present disclosure relates to a polypeptide having an acyltransferase activity or a microorganism including the same; a composition for preparing N-acetyl-L-methionine, the composition including the polypeptide or microorganism; and a method of preparing N-acetyl-L-methionine using the polypeptide or microorganism. Further, the present disclosure relates to a polynucleotide encoding the polypeptide and an expression vector including the polynucleotide.BACKGROUND ART[0002]N-Acetylmethionine, which is a derivative of methionine, has similar efficacy to methionine, but it can reduce methionine-specific flavors and can be added in a large amount compared to methionine when added to foods. In the case of animals having a rumen, when methionine is used as a feed additive, it is first used by rumen microorganisms and thus is not absorbed by the animals, whereas N-acetylmethionine is a rumen-protected amino acid that is absorbed after passing through the rumen and r...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/70C12P13/12C12N9/10C12N15/77C12N15/81
CPCC12N15/70C12P13/12C12Y203/01001C12N15/77C12N15/81C12N9/1025C12N9/1029C12R2001/125C12R2001/40C12R2001/15C12N15/63C12Y203/01C12N9/10
Inventor HONG, SO YEONPARK, JINSEUNGPARK, HYE MINCHOI, SU JINMOON, JUN OKLEE, SUNG GUNJEON, JINWOO
Owner CJ CHEILJEDANG CORP