Methods for modifying stem cell characteristics
Active Publication Date: 2012-01-31
UNIV OF CENT FLORIDA RES FOUND INC
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[0016]The invention additionally provides a method of up-regulating genes maintaining pluripotency and proliferation in cultured human bone marrow mesenchymal stem cells, the method comprising culturing the stem cells in a nutrient medium together with mouse embryonic stem cells. As above, the proposed mechanis
Problems solved by technology
This debate has caused the federal government, at least to date, to deny research funding for projects involving embryonic stem cells.
Secondly, to be considered a stem cell, it must retain the ability to morph into any other cell type.
In vitro culture of stem cells may cause a change in the biochemistry of the cells and may result in stem cells which do not behave as they might be expected to in vivo.
Accordingly, there is disagreement in the scientific community as to whether some presently maintained cell lines are truly to be considered stem cells.
These two cells types have the required characteristics of stem cells but demand quite different culture conditions and environment, without which they will quickly differentiate, losing their stem cell potential.
Somatic stem cells are typically restricted in their differential capacity so that they are able to form a limited range of more specialized cells.
In our view, this uneven segregation of cell membrane proteins is more likely a symptom of asymmetric division, rather than a cause of it.
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[0020]The present invention will now be described more fully hereinafter with reference to the accompanying drawings, in which preferred embodiments of the invention are shown. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, suitable methods and materials are described below. Any publications, patent applications, patents, or other references mentioned herein are incorporated by reference in their entirety. In case of conflict, the present specification, including any definitions, will control. In addition, the materials, methods and examples given are illustrative in nature only and not intended to be limiting. Accordingly, this invention may be embodied in many different forms and should not be constru...
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The invention provides a method of up-regulating genes in cultured human bone marrow mesenchymal stem cells, the method comprising culturing the stem cells in a nutrient medium together with cells from a limb of a limb-regenerating animal. In the method, the limb-regenerating animal is preferably a species of the family Polychrotidae. Another method of the invention provides for up-regulating genes maintaining pluripotency and proliferation in cultured human bone marrow mesenchymal stem cells by culturing the stem cells in a nutrient medium together with mouse embryonic stem cells.
Description
RELATED APPLICATION[0001]This application claims priority from provisional application Ser. No. 60 / 950,672 which was filed on 19 Jul. 2007 and which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]This invention relates to cellular differentiation and, more specifically, to methods of modifying the developmental potential of a cell and, particularly, of stem cells.BACKGROUND OF THE INVENTION[0003]Every multicellular organism contains stem cells that have the ability to differentiate into any other cell in the body. Because stem cells are essentially precursors of many other cell types, there has been much research interest in elucidating their cellular mechanisms. Imagine the promise of being able to culture replacement tissues or even whole organs from stem cells. Additionally, the potential for using stem cells to repair damaged tissues may be applicable to a wide range of disease conditions.[0004]Mammalian stem cells typically originate from three ...
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Login to View More IPC IPC(8): C12N5/07C12N5/0775C12N5/02
CPCC12N5/0663C12N2500/80C12N2506/00
InventorSUGAYA, KIMONOBUALVAREZ, ANGEL
OwnerUNIV OF CENT FLORIDA RES FOUND INC