The invention discloses an agarose gel substrate cultivated Aglaonema modestum potted plant and a preparation method thereof, and particularly relates to the technical field of cultivated Aglaonema modestum potted plant. The agarose gel substrate cultivated Aglaonema modestum potted plant includes a glass container, a high-concentration nutrient layer, a low-concentration nutrient layer and an anti-bacterial layer are sequentially arranged from bottom to top in the glass container, the low-concentration nutrient layer and the high-concentration nutrient layer are closely attached, and the anti-bacterial layer and the low-concentration nutrient layer are closely attached. Gel-cultivated Aglaonema modestum series potted plant are cleaner, a cultivation substrate is free of dust, insects andmolds, and soil and a nutrient solution are not leaked; and meanwhile, in the gel-cultivated Aglaonema modestum series potted plant, the maintenance of the Aglaonema modestum series plant is simpler and does not require subsequent supplementation of fertilizers, the problems of polluting the indoor environment with dust, breeding insects and molds by soil and difficulty in quantitatively controlling the physical and chemical properties such as water and fertilizer conditions and PH value of soil of an existing soil cultivated Aglaonema modestum potted plant are solved, and the problems that the nutrient solution is prone to being splashed and too high or too low nutrient concentration is not conducive to the survival and growth of plants are solved.