The invention discloses a preparation method of an antibacterial fresh-keeping material, which comprises the following steps: preparing a base material, preparing a coating liquid 1, coating the front surface of the base material with the coating liquid 1, curing, soaking in a sodium hydroxide aqueous solution, taking out and drying, preparing a coating liquid 2, coating centrifugal paper with the coating liquid 2, and then inversely buckling the centrifugal paper on the back surface of the base material. Through pressing and drying, the front face and the back face of a coating material are subjected to ultraviolet irradiation, cutting, edge sealing and packaging treatment, broad-spectrum antibiosis is achieved, the antibiosis rate reaches 90% or above, the fresh food curing time can be effectively prolonged, the decay rate is reduced, and the method is especially suitable for fresh food transportation and preservation under the harsh conditions of high temperature, high humidity and the like, and the loss rate is reduced. For example, for juicy peaches, the curing and softening time can be prolonged by 2-5 days, and the deterioration and decay time can be prolonged by 6-10 days; the preparation process is simple and suitable for batch production, popularization and application.