44results about How to "The reaction method is simple" patented technology

The invention discloses a method for synthesizing L-theanine through an enzyme process, and belongs to the biotechnical field. The method is characterized in that a gamma-glutamyltranspeptidase gene is obtained through chemical synthesis, a gene engineering bacterium over-expressing gamma-glutamyltranspeptidase is constructed by treating Escherichia coli as a host bacterium, glutamine and ethylamine hydrochloride having different concentrations are acted by a recombinase, and theanine is efficiently produced at a temperature of 37-50DEG C under a pH value of 9.5-10.5. The enzyme source preparation process has the advantages of simplicity, low cost, and large enzyme amount, and the theanine production method has the advantages of simplicity, high conversion rate, high output, short time and the like, and is in favor of the industrialized amplification production.

The invention discloses a preparation method for a polymer-based hierarchical porous structure interlocking microcapsule. The method comprises the steps of modifying a silicon dioxide colloid crystal template, controllably grafting a polymer by a surface triggering atom transferring free radical polymerization method and a double-bond free radical polymerization method, crosslinking a linear polymer chain section by an Friedel-Crafts crosslinking method for an external crosslinking agent, removing the silicon dioxide colloid crystal template to prepare the polymer-based hierarchical porous structure interlocking microcapsule. Due to the controllably grafted polymer layer, a certain slit is reserved between every two adjacent microcapsules, so that the mass is reduced, and more mass transfer ways are formed; by the adoption of the Friedel-Crafts crosslinking method for the external crosslinking agent, micropores and mesoporous are formed by crosslinking capsule walls made of polymer materials; the apertures of the formed holes and the crosslinking degree are adjusted according to the type of the crosslinking agent; a foundation is laid for the application; communicating windows formed among the micropores, the mesoporous and the interlocking microcapsules in the capsule walls and the slits between the microcapsules adopt classified hole interlocking structures in the mass.

The invention relates to a method for treating N-phosphonomethyl glycine (glyphosate) industrial wastewater containing high-concentration formaldehyde and formic acid. The method adopts the formaldehyde and formic acid from wastewater from sodium chlorate oxidation and wastewater treated by the method reaches biological treatment standards. Compared with the prior art, the method has the advantages that: 1, the method for preparing an aqueous solution of sodium chlorate is safe and effective and the prepared sodium chlorate has strong oxidizability; 2, the prepared aqueous solution of sodium chlorate added into the wastewater containing formaldehyde and formic acid can remove a major part of formaldehyde and formic acid in a short period, and the reaction is simple in method, quick and convenient in operation; and 3, the wastewater containing formaldehyde and formic acid is prepared into an alkali solution at a certain concentration, and chlorine is introduced into the wastewater at a high temperature to achieve a good formaldehyde and formic acid removal effect and greatly reduce COD, so the wastewater achieves a pretreatment effect and can be subjected to biological treatment to reach discharge standards.

The invention discloses a silicon-based negative electrode material for lithium ion battery, a preparation thereof, a negative electrode sheet and a secondary battery. The negative electrode materialis a metal / silicon oxide / carbon composite material C-SiOx-M with a three-dimensional porous structure prepared by a freeze-drying method. M is one or several of Fe, Mn, Co, Sn, Ti, Cu and Ni. X is isgreater than or equal to 0 and less than or equal to 2 in SiOx. The molar ratio of C: SiOx: M is (0.5-1): (1-5): 1. The negative electrode made of the negative electrode composite material shows excellent cycle stability and rate performance, and the electrical conductivity of the composite material is effectively improved.

The invention discloses a preparation method of interlocking micro-capsules based on a polymer with a high specific surface area and having a hierarchical porous structure. The method comprises the following steps: modifying a silicon dioxide colloid crystal template, performing controlled graft of a polymer chain segment by surface-initiated atom transfer radical polymerization and double-bond radical polymerization, crosslinking a linear polymer chain segment by using a friedel-crafts crosslinking method, and removing the silicon dioxide colloid crystal template, thus preparing interlocking micro-capsules based on a polymer with a high specific surface area and having a hierarchical porous structure. Through a controlled graft polymer layer, a certain gap is formed in an area which is fully filled before, thereby reducing the mass and forming more mass transfer ways. By adopting a friedel-crafts crosslinking and pore-forming technology, capsule walls made of a polymer material are cross-linked to form micro-pores and meso-pores; the material formed by adopting a friedel-crafts self-crosslinking technology is high in specific surface area, thereby greatly improving the application performance; the capsule cavities at micro-pore, meso-pore and large-pore levels, communication windows formed among interlocking micro-capsules and gaps among the micro-capsules present a hierarchical porous interlocking structure on the whole.

The invention provides a method for determining the residual quantity of sulfur dioxide in Chinese herbal medicines. The method comprises the following operation steps: (1) taking the Chinese herbal medicines to be determined, and preparing a test solution; (2) taking sulfite as a reference substance, and preparing a reference substance solution; (3) respectively taking the test solution and the reference substance solution, adding an ortho-phthalaldehyde solution and an ammonium salt solution for reaction in a buffer solution, standing after the reaction is over and stopped, and determining the residual quantity of sulfur dioxide in Chinese herbal medicines by a fluorescence derivatization method. According to the method for determining the residual quantity of sulfur dioxide in Chinese herbal medicines, the residual quantity of sulfur dioxide in Chinese herbal medicines can be accurately and fast determined, and the reaction method is simpler and more convenient and is higher in practicability while the accuracy is equivalent to that of a pharmacopoeia method.

The invention discloses a nitrogen-doped graphene nickel ferrite composite wave-absorbing material and a preparation method thereof. A nitrogen-doped reduced graphene oxide / hexagonal nickel ferrite nano composite material is prepared by taking graphene oxide (GO) as a template, taking ferric nitrate nonahydrate and nickel nitrate hexahydrate as precursors and taking hydrazine hydrate as a nitrogen-doped reagent through a simple one-step hydrothermal method. The nano composite material prepared by the invention is formed by entangling a large number of hexagonal nickel ferrite particles in nanosize by two-dimensional folded graphene, has strong electromagnetic wave absorption capability, wide absorption frequency band and small matching thickness, and can generate double absorption peaks at low frequency (3-6GHz) and high frequency (12-18GHz); and the nitrogen doping amount of graphene in the composite material can be changed by controlling the adding volume of hydrazine hydrate, meanwhile, effective attenuation of the composite material to electromagnetic waves under different wave bands can be achieved by changing the matching thickness, and the composite material has important application value in the fields of electromagnetic wave absorption and electromagnetic shielding.

The invention discloses a fluorine-containing polyarylether-based anion exchange membrane with low surface resistivity and high oxidation stability and a preparation method of the fluorine-containingpolyarylether-based anion exchange membrane. The preparation method comprises the following steps: carrying out polycondensation on tetramino bisphenol fluorene, bisphenol fluorene and decafluorobiphenyl at room temperature to synthesize a tetramino fluorine-containing polyarylether compound, and reacting the tetramino fluorine-containing polyarylether compound with a bromohexyl quaternary ammonium salt compound QA-6C to obtain a dense biquaternary ammonium salt ion side chain type fluorine-containing polyarylether compound; dissolving the obtained dense biquaternary ammonium salt ion side chain type fluorine-containing polyarylether compound into a polar aprotic solvent, and casting to form a membrane by adopting a solution casting method so as to obtain the fluorine-containing polyarylether-based anion exchange membrane with low surface resistivity and high oxidation stability. The surface resistivity of the fluorine-containing polyarylether-based anion exchange membrane under similar ion exchange capacity is lower than that of a common polyarylether-based anion exchange membrane, the oxidation stability of the fluorine-containing polyarylether-based anion exchange membrane is higher than that of the common polyarylether-based anion exchange membrane, and the fluorine-containing polyarylether-based anion exchange membrane has wide application prospects in all-vanadium redox flow batteries.

The invention relates to a method for synthesizing a Bi2O3 / (BiO)2CO3 composite photocatalytic material and belongs to the technical field of chemical industry. The method comprises the steps of weighing three kinds of chemical reagent raw materials, i.e., Na2CO3, bismuth citrate and NaOH according to a mole ratio, carrying out mixing, stirring the mixture in secondary distilled water for certain time, carrying out treatment by a programmed-heating hydrothermal process, then, carrying out repeated washing separately with deionized water and anhydrous ethanol, and carrying out baking, thereby obtaining the final product, i.e., the Bi2O3 / (BiO)2CO3 composite photocatalytic material. The surface morphology, microscopic structure and photocatalytic activity of the composite photocatalytic material are assayed, and the degradation of a variety of dyes such as rhodamine B under different light sources by the hierarchical garland-shaped Bi2O3 / (BiO)2CO3 composite photocatalytic material is greatly improved. The product is simple and practical in preparation process and stable in performance and has considerable applicability to a variety of dyes.

The invention relates to a row inter-tube falling film melt phase polycondensation reaction method. A melt prepolymer flows downwards from a film distribution plate and holes between tubes, a waterfall falling film flowing is formed between the U-shaped tubes in rows so that a melt phase polycondensation can be performed, and a melt slides down from the tubes, then condensed at the bottom of a reactor and discharged after stirring is performed to be homogenized through a stirrer. The reactor implementing the method is of an overall vertical structure and comprises a shell, a heating medium box at the upper end, a material box connected with the heating medium box, the U-shaped tubes, a bottom shell at the lower end, a stirrer and the like. The U-shaped tubes are perpendicularly suspended and arranged in rows, uniformly penetrate through holes in the film distribution plate, and the ends of the U-shaped tubes and the heating medium box are communicated to allow a heating medium to flow. The reactor has the advantages that the flowing resistance is small, no dead zone is generated, the film forming area is large, the surface updating speed is high, the standing time is uniform and controllable, and flexible production is met. The method is applicable to production of polyethylene glycol terephthalate and other polymers.

The invention relates to a substituted [1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine energetic compounds and preparation thereof, and belong to the field of organic synthesis and energetic materials. Energetic groups are successfully introduced into position 3 or / and position 6 of [1,2,4]triazolo[4,3-b][1,2,4,5]tetrazine by a substitution reaction, so that the compounds have the characteristics of highenthalpy of formation, high detonation velocity and detonation pressure, good thermal stability, low mechanical sensitivity and the like, and have potential application value in the field of energetic materials. Besides, the adopted synthesis method is simple to operate and reaction conditions are mild.

The invention relates to a preparation method of amino borane. According to the method, through taking ammonium salts and sodium borohydride as raw materials, taking an organic solvent as a reaction medium, and taking ethyl ether as a purification medium, methylamine borane is synthesized through common chemical reaction. The invention provides a method capable of synthesizing amino borane in the air, which provides great convenience for future industrial production and applications. The preparation method disclosed by the invention is simple in reaction method, low in cost and moderate in temperature; an operation of reaction can be performed at room temperature, and obtained products are high in purity and high in yield.

The invention relates to a method for synthesizing cyclic sulphoxide imine, sulfenamide and sulfamide by stereospecificty; the method comprises the following steps of: under the condition of room temperature, sulphinyl imine 4 and benzyne precursor 5 have reaction for 1-15h under the initiation of organic solvent and CsF, the cyclic sulphoxide imine 1 is generated; when the cyclic sulphoxide imine1 contains sulfonyl, the sulfonyl inside a molecule can be successfully removed under the condition of Mg / HOAc / AcONaDMF / H2O, and the cyclic sulphoxide imine 1 is obtained; under the condition of HCl(dioxane), the cyclic sulphoxide imine 1 is successfully transformed into the cyclic sulfenamide 2; and after the cyclic sulfenamide 2 is oxidized by m CPBA, the cyclic sulfamide 3 is obtained. The method has the characteristics of low cost, high yield, mild reaction condition, environmental protection, good repeatability and the like.

The invention relates to a synthetic method for a compound in the technical field of medicine and chemical engineering, and particularly relates to a novel synthetic method for tadalafil. The synthetic method comprises the steps of: 1) condensing an initial raw material which is D-tryptophan (I) and methylamino acetonitrile (II) or salt thereof to obtain a compound as shown in a formula III; 2) carrying out Pictet-Spengler cyclization reaction on the compound as shown in the formula III and heliotropin and carrying out crystallization to obtain a compound as shown in a formula V; and 3) hydrolyzing the compound V under an acidic or alkaline condition by cyano and then condensing the compound to obtain the target product tadalafil (VI). The formula is shown in the description. In the synthetic method provided by the invention, chemical raw materials which are great in toxicity, severe in environmental pollution and flammable and combustible such as thionyl chloride, chloroacetyl chloride, methylamine and the like. The novel synthetic method for tadalafil provided by the invention is simple in reaction method, convenient to operate and high in yield.

The invention provides a red phosphorus / polyimide covalent organic framework composite material with excellent flame retardant property and a preparation method thereof. The red phosphorus is improvedby utilizing the unique porous structure of the polyimide covalent organic framework material, and the interior of the red phosphorus-loaded porous polyimide covalent organic framework material is coated. The red phosphorus / polyimide covalent organic framework composite material has excellent flame retardant property, can be used as a flame retardant modified polymer organic material, does not generate molten drops in the combustion process, and has obviously higher flame retardant effect than pure red phosphorus. Furthermore, a feasible way is provided for preparing the red phosphorus flame-retardant material, and the preparation method is high in repeatability and simple in process.

The invention discloses a method for synthesizing L-theanine through an enzyme process, and belongs to the biotechnical field. The method is characterized in that a gamma-glutamyltranspeptidase gene is obtained through chemical synthesis, a gene engineering bacterium over-expressing gamma-glutamyltranspeptidase is constructed by treating Escherichia coli as a host bacterium, glutamine and ethylamine hydrochloride having different concentrations are acted by a recombinase, and theanine is efficiently produced at a temperature of 37-50DEG C under a pH value of 9.5-10.5. The enzyme source preparation process has the advantages of simplicity, low cost, and large enzyme amount, and the theanine production method has the advantages of simplicity, high conversion rate, high output, short time and the like, and is in favor of the industrialized amplification production.

The invention relates to a method for treating N-phosphonomethyl glycine (glyphosate) industrial wastewater containing high-concentration formaldehyde and formic acid. The method adopts the formaldehyde and formic acid from wastewater from sodium chlorate oxidation and wastewater treated by the method reaches biological treatment standards. Compared with the prior art, the method has the advantages that: 1, the method for preparing an aqueous solution of sodium chlorate is safe and effective and the prepared sodium chlorate has strong oxidizability; 2, the prepared aqueous solution of sodium chlorate added into the wastewater containing formaldehyde and formic acid can remove a major part of formaldehyde and formic acid in a short period, and the reaction is simple in method, quick and convenient in operation; and 3, the wastewater containing formaldehyde and formic acid is prepared into an alkali solution at a certain concentration, and chlorine is introduced into the wastewater at ahigh temperature to achieve a good formaldehyde and formic acid removal effect and greatly reduce COD, so the wastewater achieves a pretreatment effect and can be subjected to biological treatment toreach discharge standards.

The invention discloses a platinum sulfide protein nanoparticle with a near-infrared photothermal effect and a multi-mode imaging function as well as a preparation method and application of the platinum sulfide protein nanoparticle. By carrying out formulation screening and process limitation, the platinum sulfide protein nanoparticle with the near-infrared photothermal effect and the multi-mode imaging function is prepared in a water phase, has an ultra-small particle size and good stability, tumor targeting property and photothermal effect and is integrated with near-infrared imaging, CT imaging, photoacoustic imaging and thermal imaging functions, the high-sensitivity and high-resolution precise localization of tumors is realized, an efficient photothermal effect is generated under thestimulation of near-infrared light, tumor cells are killed through thermal ablation, and the efficient, safe, visual and accurate treatment of the tumors is realized; and the platinum sulfide proteinnanoparticle has potential to be further applied to the clinic.

The invention provides a method for producing rhodioloside by enzymatic conversion and fermentation with apple beta-glucosidase as a novel catalyst. The apple beta-glucosidase is prepared with high-efficiency by a gene recombination technique; 50 mM-1000 mM of tyrosol and 10 mM-500 mM of glucose are added and subjected to reaction for 20-50 h at the temperature of 37-50 DEG C, and the rhodiolosideis obtained.

The invention relates to the field of lithium battery electrolytic solution, and particularly discloses a preparation process of lithium difluoro (oxalato) borate. The preparation method comprises the following steps: adding raw materials for reaction according to a ratio of the metering number of a reaction formula (1) (Me)2SiCl2+H2C2O4+LiBF4->LiBC2O4F2+(Me)2SiF2+2HCl, and filtering after reaction; performing concentration; and crystallizing and drying to obtain the product. The preparation method provided by the invention has the advantage that the prepared lithium difluoro (oxalato) borate is high in purity.

The invention relates to the field of enzyme engineering, and discloses a large-island saline-alkali bacillus for producing cyclodextrin glucosyltransferase and application of the large-island saline-alkali bacillus. According to the invention, a large island saline-alkali bacillus (WahahaMZJ-1) for expressing cyclodextrin glucosyltransferase is screened, and the preservation number of the large island saline-alkali bacillus is CGMCC (China General Microbiological Culture Collection Center) No.23164. The invention also discloses a preparation method of the large island saline-alkali bacillus. Compared with the existing commercial similar enzymes, the glucosyl stevioside is generated by carrying out enzyme reaction on the obtained enzyme, and the contents of RebD, RebD isomeride, RebM and RebM isomeride in the converted product are high, so that the taste is better; meanwhile, the method has the advantages of simple reaction method, low energy consumption, low material cost, high conversion efficiency and the like, and lays a foundation for industrial large-scale production.

The invention discloses an amino acid amide compound and a preparation method thereof. Amino acid protected by FMOC and an acyl chloride reagent react to produce amino acid acyl chloride which is dissolved into a solvent with an appropriate polarity and reacts with a simple compound with primary amine and secondary amine so that the prepared amino acid amide compound can precipitate once forming in the reaction process, thereby reducing the contact of the FMOC and the amine; the whole process is short in the reaction time and fast in speed, thereby preventing the de-protection and realizing that the amino acid protected by the FMOC reacts with the simple amine on the premise without removing protective group; and the yield is up to more than 93 percent. The reaction method is simple, and has high efficiency and clean products as well as easy post treatment.

The invention provides a method for using apple β-glucosidase as a novel catalyst to produce and prepare salidroside through enzymatic transformation and fermentation. Using gene recombination technology to prepare high-efficiency apple β-glucosidase, add 50mM-1000mM tyrosol, 10mM-500mM glucose, and react at 37-50°C for 20-50h. Obtain salidroside.

The invention provides a preparation method of a 2,5-dialkoxyl dihydrofuran compound. The preparation method comprises the following steps: adding a furan compound shown by a formula I, alkyl alcohol shown by a formula III and an acid-binding agent into a reactor, stirring to react with chlorine, keeping the reaction temperature to be (-25)-25 DEG C, and reacting to obtain a 2,5-dialkoxyl dihydrofuran compound shown by a formula II, wherein in the formulas, R1, R2, R3 and R4 are independent hydrogen, halogen and C1-C5 alkyl, and R1 is a C1-C12 alkyl. The method provided by the invention is less in investment, low in energy consumption and high in efficiency; the reaction method is simple, and the operation is convenient and easy to implement; the raw materials are low in price, easy to obtain, and low in production cost; a byproduct is salt and can meet requirements on environmental protection; and the preparation method is suitable for performing industrial large-scale production of the 2,5-dialkoxyl dihydrofuran compound. Particularly, when a phase transfer catalyst is added into the method provided by the invention, the method also has the advantage that the yield of a target product is obviously improved.

The invention relates to a method for synthesizing a short-chain flavor ester with cutinase and belongs to the technical field of enzyme engineering and fermentation engineering. According to the method, pichia pastoris serves as a host bacterium to build a genetically engineered bacterium of overexpressed cutinase, the genetically engineered bacterium is induced to generate cutinase through fermentation, and enzyme activity of cutinase in supernatant of fermentation liquor reaches 420 U / mL. The recombinant enzyme is further used to produce the short-chain flavor ester in an organic phase, Chinese rice wine aging acceleration and baijiu solid fermentation. The method has the advantages that an enzyme source preparation method is simple, cost is low, enzyme quantity is large, a short-chain flavor ester production method is simple, the conversion rate is high, the yield is high and time is short, and industrial enlarged production is facilitated.

The invention provides a method for determining the residual quantity of sulfur dioxide in Chinese herbal medicines. The method comprises the following operation steps: (1) taking the Chinese herbal medicines to be determined, and preparing a test solution; (2) taking sulfite as a reference substance, and preparing a reference substance solution; (3) respectively taking the test solution and the reference substance solution, adding an ortho-phthalaldehyde solution and an ammonium salt solution for reaction in a buffer solution, standing after the reaction is over and stopped, and determining the residual quantity of sulfur dioxide in Chinese herbal medicines by a fluorescence derivatization method. According to the method for determining the residual quantity of sulfur dioxide in Chinese herbal medicines, the residual quantity of sulfur dioxide in Chinese herbal medicines can be accurately and fast determined, and the reaction method is simpler and more convenient and is higher in practicability while the accuracy is equivalent to that of a pharmacopoeia method.

The invention discloses a nano-composite material based on Ce-based amorphous alloy and its preparation method and its application in treating dye wastewater. After reacting Ce-based amorphous alloy with dilute acid, adding alkali to settle to obtain suspension, Then, the suspension was subjected to hydrothermal treatment to obtain a nanocomposite material based on Ce-based amorphous alloy. The nanocomposite material of the present invention can be in dark normal temperature and pressure, and do not add any chemicals (H 2 o 2 , PS / PMS or O 3 ) and energy, it has a high removal rate for dye wastewater.

The invention discloses a preparation method of interlocking micro-capsules based on a polymer with a high specific surface area and having a hierarchical porous structure. The method comprises the following steps: modifying a silicon dioxide colloid crystal template, performing controlled graft of a polymer chain segment by surface-initiated atom transfer radical polymerization and double-bond radical polymerization, crosslinking a linear polymer chain segment by using a friedel-crafts crosslinking method, and removing the silicon dioxide colloid crystal template, thus preparing interlocking micro-capsules based on a polymer with a high specific surface area and having a hierarchical porous structure. Through a controlled graft polymer layer, a certain gap is formed in an area which is fully filled before, thereby reducing the mass and forming more mass transfer ways. By adopting a friedel-crafts crosslinking and pore-forming technology, capsule walls made of a polymer material are cross-linked to form micro-pores and meso-pores; the material formed by adopting a friedel-crafts self-crosslinking technology is high in specific surface area, thereby greatly improving the application performance; the capsule cavities at micro-pore, meso-pore and large-pore levels, communication windows formed among interlocking micro-capsules and gaps among the micro-capsules present a hierarchical porous interlocking structure on the whole.