Method for promoting salt and drought tolerance of maize and wheat by combining betAíóNHX1íóPPase gene and transgene technology

Abstract

This invention discloses a method for improving the salt resistance and drought resistance of the corn and wheat by aggregating the beta, NHX1, PPase genes with the transfer genes, the steps as follows: regrouping the solution film natrium hydrogen counter-operating protein gene NHX1, the solution film tar phosphatase gene PPase and the Cholinesterase dehydrogenase gene from the escherichia colim to the plant expression carrier, then adding the corn or wheet cells and make them express efficiently, then producing the transfer gene plant; screening out the transfer gene pure cell that has apparently higher salt resistance and drought resistance from the transfer gene plant and its offspring; getting the transfer gene plant with the three object genes through twice tranfering the transfer gene pure cell or making the plant with the different transfer genes mate mutully, then sceening out the ones that have better salt resistance and drought resistance in their offsprings, and making them mute and purify by themselves to creat the new corn and wheat seeds with the salt resistance and drought resistance; or making use of the transfer polymeric material to produce the mixing seeds directly.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, and specifically relates to a method and application for improving the salt and drought tolerance of corn and wheat by transgenic polymerization of betA, NHX1 and PPase genes. Background technique [0002] Among all kinds of natural adversities, the impact of drought on the world's crop production ranks first, and its harm is equivalent to the sum of other natural disasters. Salinization is also a major environmental factor affecting crop yields. About half of my country's land area is in arid and semi-arid areas. Even major agricultural areas in non-arid regions are occasionally hit by drought. Among the 1.6 billion mu of arable land in my country, there are more than 100 million mu of saline-alkali land, and about 1 billion mu of medium and low-yield fields. Most of these low-yielding fields are also due to drought and salinity. In addition, there are 300 million mu of saline...

AI Technical Summary

Problems solved by technology

Research in this area is still in its infancy at home and abroad, and the best technical route and method have not yet been determined.