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Dog intravenous injection albumin, its preparing method and dog intravenous injection albumin preparation

An albumin and preparation technology, which is applied to the canine intravenous albumin preparation, its preparation, the field of canine intravenous albumin injection, can solve the problem that albumin cannot be used for intravenous injection, etc., and achieves low cost, stable performance, and good finished product purity. Effect

Active Publication Date: 2008-03-26
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some inventors have studied the method of extracting protein from animal blood, such as patent application 9410410.8. However, due to technical reasons, the albumin developed by it cannot be applied to intravenous injection
At present, there is no literature report on the research and application of intravenous immunoglobulin for dogs

Method used

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  • Dog intravenous injection albumin, its preparing method and dog intravenous injection albumin preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Extraction of canine albumin by rivanol method

[0038] 1) Melt slurry and adjust pH

[0039] Melt 200,000 ml of plasma that meets the raw material requirements quickly at 37°C, and put it into a reaction tank under a sterile environment; slowly add Na at a concentration of 1% while stirring 2 CO 3 , adjust the pH to 8.5±0.1.

[0040] 2) Complexation and dissociation

[0041] In the stirring state, add 2% rivanol equal volume (200,000 milliliters) to the blood plasma in the reaction tank, stop stirring after adding, and let it stand for 2 hours.

[0042] Take out the supernatant after complexation in the reaction tank for the separation of immunoglobulin, and leave the precipitated part in the tank; start the agitator, and use the PP6 pump to add NaCl, HCl solution and sodium octanoate solution into the tank successively; the mixture is stirred More than 2 hours or overnight;

[0043] Add 0.6% NaCl, 0.2% sodium octanoate and 4% 0.5 mol HCl at a final con...

Embodiment 2

[0050] Embodiment 2: adopt low-temperature ethanol method to extract canine albumin

[0051] Described low-temperature ethanol method comprises the following steps:

[0052] 1).Melting slurry and adding ethanol

[0053]Melt 200,000 ml of plasma that meets the raw material requirements quickly at 37°C, and put it into a reaction tank under a sterile environment; gradually cool down to 0°C while stirring, and adjust the pH to 7.2 with an acetate buffer with a pH of 4 ±0.2; while stirring, add 53.3% ethanol pre-cooled at -15°C--20°C until the final concentration of ethanol volume is 8% (add ethanol slowly, based on 100,000 ml of plasma, add 400-500ml per minute, 2 -3 hours to complete the addition), the temperature was maintained at -2°C--3°C, after the addition was completed, continue to stir for 1 hour, and let it stand for 2 hours.

[0054] 2). Centrifuge to remove impurities

[0055] Use G142 continuous centrifuge, 13000r, centrifuge several times, discard the precipitate ...

Embodiment 3

[0065] Example 3: Preparation of albumin preparation for dog intravenous injection--intravenous injection solution with albumin content of 1% to 25%

[0066] The albumin solution with a concentration of 1 to 20% extracted in Example 1 and Example 2 is followed by the following operations to obtain an albumin preparation for intravenous injection:

[0067] 5) Inspection and addition of reagents:

[0068] Take the concentrated sample and submit it for inspection to determine the content of albumin; add sodium octanoate at 0.16mmol / g albumin; add NaCl at 160mmol / L albumin solution; adjust to pH 7.0±0.2.

[0069] 6) Inactivation:

[0070] Put the albumin solution into a stainless steel milk jug and seal it. Each batch of products must be heated at 60±0.5°C for 10 hours. The inactivation treatment can be carried out before sterile filtration or within 24 hours after aliquoting.

[0071] 7) Batch:

[0072] Products mixed in the same manufacturing process and in the same contain...

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Abstract

Blood plasma or blood serum from health dog is separated in low temperature ethanol process or rivanol process to prepare dog blood albumin with pH value of 6.4-7.4 and purity not lower than 95 % and capable of being used for dog's intravenous injection. The dog blood albumin may be added with proper amount of stabilizer to prepare intravenous albumin injection with albumin content of 1-25 % or albumin injection with albumin content of 1-25 % and glucose in 3-10 % or glucose brine in 3-5 %.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to albumin for canine intravenous injection, a preparation method thereof and an albumin preparation for canine intravenous injection. Background of the invention [0002] Pets are becoming more and more important in people's daily life, and pet health care has become an important topic. Canine blood products are biological products separated and extracted from canine plasma, also known as plasma derivatives. Such products can be used for the treatment or supplementation of canine diseases. [0003] One of the above-mentioned biological products is albumin. As a solute, albumin can reduce the potential energy of water molecules in the solution. 1 gram of albumin can keep 18 grams of water in blood vessels, and the resulting osmotic pressure is equivalent to 20 milliliters of plasma or 40 milliliters of whole blood. Blood, and certain ions (such as calcium, copper, zinc and other dival...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/76A61K38/38A61K35/16A61P7/00A61P7/10A61P1/16A61P13/12
Inventor 章金刚王建国赵宝全吕茂民杨姝孙文种熊景峰朱晓霞
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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