Process for separating purifying actived tea polyphenols

Abstract

The invention discloses a method to separate and purify active tea polyphenol. The feature is that washing chlorine ball by distilled water, swelling by solvent, drying; weighing chlorine ball for 1-4 portions, collagen or collagen polypeptide 5-20 portions, buffer solution that pH value is 8-12 10-40 portions, adding to reactor; reacting for 6-18 hours at 50-90 degree centigrade, filtering acting liquid, washing and drying as column filling material; adding the column filling material into chromatography column, the distilling liquid flowing passing the column, after saturating with each other, using water solution that the pH value is 2-6 to wash column, removing filling material by 50-90 edible alcohol, recycling alcohol, drying, the active tea polyphenol product that has purity over 95%, caffeine less than 1%.

Description

1. Technical field [0001] The invention relates to a method for separation and purification of tea polyphenols, which belongs to the field of separation and processing of natural products. 2. Background technology [0002] Since the systematic research on tea polyphenols by H.Roberts et al. in the 1950s, many functions of tea polyphenols have been discovered one after another. With the deepening of research and the continuous discovery of new functions of tea polyphenols, people's interest in tea polyphenols is increasing day by day. A large number of studies have shown that tea polyphenols are not only a natural non-toxic antioxidant, but also an ideal natural medicine. It has good effects in cancer, tumor suppression, and cardiovascular disease prevention and treatment. [0003] The production and sales of tea polyphenols have a history of more than ten years at home and abroad. Tea polyphenols are mainly used in the following aspects: anti-tumor drugs; cardiovascular he...

AI Technical Summary

Problems solved by technology

The solvent method has a simple process, but the production cycle is long and the temperature is high. Tea polyphenols are easy to oxidize and lose their activity. The content and activity of the prepared tea polyphenols are low, and a large amount of organic solvents are used in the production process. The residue and extraction rate are low, and the environmental pollution is relatively large; the precipitation method is greatly restricted due to the high residue of metal ions in the product, and this method has been eliminated; the adsorption method has simple process, convenient operation, mild production conditions, and the production process The organic solvent used in the method is mainly ethanol solution, and the tea polyphenols produced have high content and good biological activity, but the column packing of the adsorption method has poor selectivity for the adsorption of tea polyphenols, and gradient elution is required to remove impurities , so the yield of tea polyphenols is low

[0005] At present, my country basically adopts the solvent method to produce tea polyphenols. The tea polyphenols produced by this process have low activity and high residual amount of toxic reagents. Polyphenol products are difficult to sell and there is a large backlog. At the same time, the solvent method pollutes the environment greatly, which is not in line with the development direction of "green chemistry".

Although column chromatography has some shortcomings, the tea polyphenols produced by this method have high activity and the process is environmentally friendly. As long as it is properly improved, a new green process that meets the needs of active tea polyphenols production can be developed