Process for separating purifying actived tea polyphenols
A technology for separating and purifying tea polyphenols, applied in the field of separation and processing of natural products, can solve the problems of long production cycle, large environmental pollution, low activity of tea polyphenols, etc., and achieve the effect of safe production process
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Embodiment 1
[0018] 1. Rinse the chlorine ball with distilled water, swell it with chloroform, and dry it; weigh 10 grams of the dried chlorine ball, 50 grams of collagen and 100 grams of buffer solution with pH=8-12 and add it to the reactor; React at 50°C for 6 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.
[0019] 2. Weigh 10 grams of the above-mentioned column filler, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column filler with an aqueous solution of pH=2-6, Then use 50% edible ethanol to elute the filler, recover the ethanol, and freeze-dry to obtain an active tea polyphenol product with a purity > 95% and caffeine < 1%, which is stored at low temperature and protected from light.
Embodiment 2
[0021] 1. Rinse the chlorine ball with distilled water, swell it with ethylene dioxide, and dry it; weigh 40 grams of the dried chlorine ball, 200 grams of collagen polypeptide and 400 grams of buffer solution with pH=8-12 and add them to the reactor; React at a temperature of 70° C. for 18 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.
[0022] 2. Weigh 10 grams of the material synthesized above, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column packing with an aqueous solution of pH=2-6 , and then use 95% edible ethanol to elute the filler, recover the ethanol, and spray dry to obtain the active tea polyphenol product with a purity > 95% and caffeine < 1%, and store it at low temperature and avoid light.
Embodiment 3
[0024] 1. Rinse the chlorine ball with distilled water, then swell it with chloroform, and dry it; weigh 30 grams of the dried chlorine ball, 100 grams of collagen and 200 grams of buffer solution with pH=8-12 and add them to the reactor; React at 80°C for 10 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.
[0025] 2. Weigh 10 grams of the material synthesized above, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column packing with an aqueous solution of pH=2-6 , and then use 80% edible ethanol to elute the filler, recover the ethanol, and freeze-dry to obtain an active tea polyphenol product with a purity > 95% and caffeine < 1%, and store it at low temperature and avoid light.
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