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Process for separating purifying actived tea polyphenols

A technology for separating and purifying tea polyphenols, applied in the field of separation and processing of natural products, can solve the problems of long production cycle, large environmental pollution, low activity of tea polyphenols, etc., and achieve the effect of safe production process

Active Publication Date: 2006-06-28
谢君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent method has a simple process, but the production cycle is long and the temperature is high. Tea polyphenols are easy to oxidize and lose their activity. The content and activity of the prepared tea polyphenols are low, and a large amount of organic solvents are used in the production process. The residue and extraction rate are low, and the environmental pollution is relatively large; the precipitation method is greatly restricted due to the high residue of metal ions in the product, and this method has been eliminated; the adsorption method has simple process, convenient operation, mild production conditions, and the production process The organic solvent used in the method is mainly ethanol solution, and the tea polyphenols produced have high content and good biological activity, but the column packing of the adsorption method has poor selectivity for the adsorption of tea polyphenols, and gradient elution is required to remove impurities , so the yield of tea polyphenols is low
[0005] At present, my country basically adopts the solvent method to produce tea polyphenols. The tea polyphenols produced by this process have low activity and high residual amount of toxic reagents. Polyphenol products are difficult to sell and there is a large backlog. At the same time, the solvent method pollutes the environment greatly, which is not in line with the development direction of "green chemistry".
Although column chromatography has some shortcomings, the tea polyphenols produced by this method have high activity and the process is environmentally friendly. As long as it is properly improved, a new green process that meets the needs of active tea polyphenols production can be developed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Rinse the chlorine ball with distilled water, swell it with chloroform, and dry it; weigh 10 grams of the dried chlorine ball, 50 grams of collagen and 100 grams of buffer solution with pH=8-12 and add it to the reactor; React at 50°C for 6 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.

[0019] 2. Weigh 10 grams of the above-mentioned column filler, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column filler with an aqueous solution of pH=2-6, Then use 50% edible ethanol to elute the filler, recover the ethanol, and freeze-dry to obtain an active tea polyphenol product with a purity > 95% and caffeine < 1%, which is stored at low temperature and protected from light.

Embodiment 2

[0021] 1. Rinse the chlorine ball with distilled water, swell it with ethylene dioxide, and dry it; weigh 40 grams of the dried chlorine ball, 200 grams of collagen polypeptide and 400 grams of buffer solution with pH=8-12 and add them to the reactor; React at a temperature of 70° C. for 18 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.

[0022] 2. Weigh 10 grams of the material synthesized above, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column packing with an aqueous solution of pH=2-6 , and then use 95% edible ethanol to elute the filler, recover the ethanol, and spray dry to obtain the active tea polyphenol product with a purity > 95% and caffeine < 1%, and store it at low temperature and avoid light.

Embodiment 3

[0024] 1. Rinse the chlorine ball with distilled water, then swell it with chloroform, and dry it; weigh 30 grams of the dried chlorine ball, 100 grams of collagen and 200 grams of buffer solution with pH=8-12 and add them to the reactor; React at 80°C for 10 hours, filter the reaction solution, wash the reaction product with distilled water, and use it as column packing after drying.

[0025] 2. Weigh 10 grams of the material synthesized above, fill it in a chromatographic column, and then flow the extract of active tea polyphenols through the chromatographic column. After the interaction is saturated, wash the column packing with an aqueous solution of pH=2-6 , and then use 80% edible ethanol to elute the filler, recover the ethanol, and freeze-dry to obtain an active tea polyphenol product with a purity > 95% and caffeine < 1%, and store it at low temperature and avoid light.

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PUM

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Abstract

The invention discloses a method to separate and purify active tea polyphenol. The feature is that washing chlorine ball by distilled water, swelling by solvent, drying; weighing chlorine ball for 1-4 portions, collagen or collagen polypeptide 5-20 portions, buffer solution that pH value is 8-12 10-40 portions, adding to reactor; reacting for 6-18 hours at 50-90 degree centigrade, filtering acting liquid, washing and drying as column filling material; adding the column filling material into chromatography column, the distilling liquid flowing passing the column, after saturating with each other, using water solution that the pH value is 2-6 to wash column, removing filling material by 50-90 edible alcohol, recycling alcohol, drying, the active tea polyphenol product that has purity over 95%, caffeine less than 1%.

Description

1. Technical field [0001] The invention relates to a method for separation and purification of tea polyphenols, which belongs to the field of separation and processing of natural products. 2. Background technology [0002] Since the systematic research on tea polyphenols by H.Roberts et al. in the 1950s, many functions of tea polyphenols have been discovered one after another. With the deepening of research and the continuous discovery of new functions of tea polyphenols, people's interest in tea polyphenols is increasing day by day. A large number of studies have shown that tea polyphenols are not only a natural non-toxic antioxidant, but also an ideal natural medicine. It has good effects in cancer, tumor suppression, and cardiovascular disease prevention and treatment. [0003] The production and sales of tea polyphenols have a history of more than ten years at home and abroad. Tea polyphenols are mainly used in the following aspects: anti-tumor drugs; cardiovascular he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/04A61P35/00A61P9/00A61P3/10A61P31/12
Inventor 谢君陈荣义刘英菊刘畅刘小军王登良王若涛王余霞
Owner 谢君
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