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Production of pectinid with thelykaryon development characteristic and construction of pectinid pure system

A gynogenesis and scallop technology, applied in the field of shellfish seed production, can solve the problems of low survival rate of offspring, early embryonic death, gene mutation, etc., and achieve the effect of facilitating genetic research and shortening time.

Inactive Publication Date: 2008-09-24
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to treatments that inactivate sperm chromosomes, often resulting in genetic mutations and early death of embryos and poor offspring survival
The use of these methods to produce shellfish with gynogenetic characteristics cannot meet the needs of shellfish genetics research and shellfish breeding and production, and there are no reports of successfully breeding diploid adult scallops with gynogenetic characteristics in current research

Method used

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  • Production of pectinid with thelykaryon development characteristic and construction of pectinid pure system

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Under artificial control conditions, female Chlamys scallops were crossed with male Scallops grandis, and 18% of the progeny larvae with gynogenetic characteristics were produced in the offspring, and 30% to 70% of these progeny larvae could grow normally. A type cytometer was used to screen live chromosomes, and individuals with 38 pairs of chromosomes were selected for culture. After these scallops with 38 pairs of chromosomes were further raised for more than 2 to 3 cm, 10 pairs of microsatellite SSR tests were performed, and it was found that 100% of the progeny scallops had gynogenetic characteristics; Further hybridize with Huagui scallop, and screen live chromosomes of the hybrid progeny by flow cytometry, select individuals with 38 pairs of chromosomes for cultivation, and pass 10 pairs of microsatellite SSR detection sites after breeding to 2-3 cm. It is found that the allelic purity of scallops after hybridization is higher, and then these scallops are bred to...

Embodiment 2

[0021] Under artificial control conditions, female Chlamys scallops were crossed with male Ezo scallops, and 20% of the progeny larvae with gynogenetic characteristics were produced in the offspring, and 40% to 80% of these progeny larvae could grow normally. Screen live chromosomes by flow cytometry, and select individuals with 38 pairs of chromosomes for culture. These scallops with 38 pairs of chromosomes were further raised for more than 2 to 3 cm, and then detected by 10 pairs of microsatellite SSR, and it was found that 100% of the progeny scallops had gynogenetic characteristics; Crossbreed with Ezo scallop, screen live chromosomes of the hybrid progeny by flow cytometry, select individuals with 38 pairs of chromosomes for culture, and pass 10 pairs of microsatellite SSR detection sites after breeding to 2 to 3 cm. It is found that the allelic purity of scallops after hybridization is higher, and then these scallops are bred to sexual maturity, and the male and female i...

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Abstract

A method for culturing the scallop with gynogenesis characteristics and fast creating a pure scallop line includes such steps as choosing different kinds of health scallops with mature sex gland, regulating the mature periods of male and female parents for synchronizing their sexual maturities, drying in air for stimulating the generation of spermatozoa and ova, fertilizing, culturing the larvae with gynogenetic characteristics, and screening the young scallops with 38 normal chromosomes.

Description

technical field [0001] The invention relates to shellfish seed production technology, in particular to a method for producing scallops with gynogenetic characteristics and quickly establishing pure lines of scallops. Background technique: [0002] Gynogenesis has a broad application prospect in shellfish breeding. First, it can quickly obtain pure lines of shellfish. The genetic basis of artificial gynogenesis mainly comes from the female parent, so its offspring have high homozygosity. After nuclear development, its progeny can be used as pure lines for breeding and hybrid production. In combination breeding, it takes a long time to obtain a stable variety due to the continuous separation of offspring. Using the first generation of hybrid seeds or the second generation of hybrid seeds for gynogenesis, only two or three generations can be used to obtain stable strains, which greatly reduces time for breeding. And shellfish with gynogenetic characteristics are of great sign...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A01K67/00A61D19/00
CPCY02A40/81
Inventor 包振民陈刚胡景杰汪小龙潘杰
Owner OCEAN UNIV OF CHINA