Carboxy methyl schizophllan polysacharide preparing method and its use for use in cosmetics and anticancer drugs

A technology for carboxymethyl schizophyllan and schizophyllan, which is used in cosmetics and anti-tumor drugs. The field of preparation of carboxymethyl schizophyllan can solve the problem that the molecular weight and properties of the product are difficult to control, the vibrating rod is easy to corrode, and the energy consumption and noise, to achieve the effect of improving moisture absorption and moisturizing effect, easy to dissolve, and low cost

Active Publication Date: 2008-12-03
GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modification of Schizophyllan by physical ultrasound has problems such as long action time, low efficiency, high energy consumption and noise, and easy corrosion of the vibrating rod.
However, although general chemical modification has the advantages of fast reaction and low energy consumption, it is difficult to control the molecular weight and properties of the product.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 10g Schizophyllan, add 100mL isopropanol to a 500mL reactor, stir at 100r / min for 60min; add 100mL 30% sodium hydroxide solution, stir at 100r / min for 2h; add 25ml 4mol / L chloroacetic acid solution, react at 60°C 3h, the stirring speed is 300r / min; after the reaction, use 10.0mol / L acetic acid to adjust the pH to 6, and the reaction solution is desalted through a 50KD ultrafiltration membrane, concentrated and freeze-dried to obtain white spongy carboxymethyl schizophyllan CM1 .

Embodiment 2

[0027] Add 6g of Schizophyllan, add 30mL of water, stir at 100r / min for 30min; add 6mL of 20% sodium hydroxide solution, stir at 300r / min for 25min; add 6mL of 3mol / L chloroacetic acid solution, react at 100°C for 1h, and stir at 100r / min min; after the reaction, the pH was adjusted to 7 with 10.0 mol / L acetic acid; the reaction solution was desalted through a 30KD ultrafiltration membrane, concentrated and spray-dried to obtain carboxymethyl schizophyllan CM2 in the form of white powder.

Embodiment 3

[0029] Add 10g of Schizophyllan, add 250mL of acetone, stir at 100r / min for 40min; add 100mL of 25% sodium hydroxide solution to a 500mL reactor, stir at 200r / min for 1h; add 35ml of 2mol / L chloroacetic acid solution, react at 45°C for 2h, The stirring speed was 500r / min; after the reaction, the pH was adjusted to 6.5 with 10.0 mol / L acetic acid; the reaction solution was desalted through a 100KD ultrafiltration membrane, concentrated and freeze-dried to obtain white spongy carboxymethyl schizophyllan CM3.

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PUM

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Abstract

The invention discloses a carboxymethyl crack pleat polysaccharide preparation method and its application in cosmetic and antineoplastic. Neutral crack pleat polysaccharide is transformed into carboxylic acid sodium salt with negative electric charge by its carboxy methylation in the invention. And this not only can improve its solubility, but also can keep its good rheological behavior and antineoplastic activity. The invention uses the chemical agent to substitute the carboxyl of the polysaccharide, and controls molecular weight and degree of substitution of the carboxymethyl crack pleat polysaccharide by reaction condition to make it have good solubility and antineoplastic activity. And it can apply in cosmetic, antineoplastic, food, and so on.

Description

technical field [0001] The invention belongs to the field of carboxymethyl schizophyllan, in particular to a preparation method of carboxymethyl schizophyllan and its application in cosmetics and antitumor drugs. Background technique [0002] Schizophyllan is a neutral exopolysaccharide produced by submerged fermentation of the medicinal fungus Schizophyllum commune Fries. Schizophyllan has a β-(1-3)-D glucan structure with β-(1-6) glucoside branches, and every 3 glucose units on the main chain produce a branch. Because of its good physical and chemical properties (such as rheological properties) and physiological activities (such as anti-tumor, anti-aging), it has attracted the attention of researchers at home and abroad. However, during the research process, we found that due to the large molecular weight and unique triple helix structure of Schizophyllan, the dried Schizophyllan dissolves slowly in water, and it is difficult to dissolve completely, even with DMSO (dimeth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P35/00
Inventor 郭祀远周林李琳郑必胜蔡妙颜王兆梅肖凯军魏东
Owner GUANGDONG MARUBI BIOLOGICAL TECH CO LTD
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