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Relativity of voltage controlling sodium pass 7 type alpha subunit gene with primary hypertension

A technology of voltage regulation and hypertension, applied in the fields of molecular biology and medicine, can solve problems such as uncertainty

Inactive Publication Date: 2009-06-03
SHANGHAI INST OF HYPERTENSION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has not been determined which genes in this region are EH susceptibility genes

Method used

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  • Relativity of voltage controlling sodium pass 7 type alpha subunit gene with primary hypertension
  • Relativity of voltage controlling sodium pass 7 type alpha subunit gene with primary hypertension
  • Relativity of voltage controlling sodium pass 7 type alpha subunit gene with primary hypertension

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0145] 1 Objects and methods

[0146] 1.1 Research object

[0147] 1.1.1 Chinese SNP discovery and confirmation samples

[0148] In order to improve the representativeness and effectiveness of SNP detection, DNA samples were taken from 30 subjects from different regions of my country (Shandong, Shaanxi, Fujian, Guangdong, Guangxi, Shanghai, Lhasa, Yunnan, Jilin, Inner Mongolia, Guizhou, Sichuan and Xinjiang, etc. ) of different nationalities (Han, Zhuang, Tibetan, Brown, Korean, Dai, Mongolian, Miao, Yi, Uighur, etc.).

[0149] 1.1.2 Preliminary research samples of SNP typing and association analysis

[0150] All subjects were of Han nationality. The essential hypertension group (EH group) and the normotensive group (NT group) had 96 cases each, both from Shanghai, China, and had no relationship with each other. The EH group consisted of hypertensive patients who were diagnosed in the Hypertension Department of Shanghai Ruijin Hospital or in the general survey in Shanghai, ...

Embodiment 2

[0191] Essential Hypertension Susceptibility Detection Kit

[0192] A kit is prepared which contains:

[0193] Name Sequence (5′→3′) Number Concentration

[0194] Forward primer cct gga gcc tac aat ctc tga SEQ ID NO: 47 dry powder 2 OD

[0195] Reverse primer gaa agg tgt cat gta cca aca atg SEQ ID NO: 48 dry powder 2 OD

[0196] PCR reaction solution PCR reaction buffer containing Taq enzyme, dNTP, magnesium ion

[0197] Enzyme Reaction Solution BclI Endonuclease and Reaction Solution

[0198] Take 3ml of blood from the patient to be tested, and use a conventional method (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the hypertension detection kit to 1 μmol / μl, and use the extracted DNA as a template to carry out PCR reaction with the provided primers. The PCR product was digested with BclI endonuclease. The amplification product containing T at the 153rd position can be cut by BclI, while the PCR product containing G at the 153rd positi...

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Abstract

The invention discloses a method for detecting the susceptibility of essential hypertension, which includes detecting whether there is a variation in the voltage-regulated sodium channel type 7 alpha subunit gene SCN7A, transcript and / or protein of an individual compared with normal, and whether there is a variation It shows that the individual is more likely to suffer from essential hypertension than the normal population. The invention also discloses a corresponding detection kit.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (single nucleotide polymorphism, SNP) of the human voltage-regulated sodium channel type 7 subunit gene (sodium channel, voltage-gated, type VII, alpha polypeptide, SCN7A) and its relationship with the original association with hypertension. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Essential hypertension (essential hypertension, EH) is the most common cardiovascular disease, its incidence rate is increasing year by year, and can cause serious heart, brain, kidney complications, is a major risk factor for stroke and coronary heart disease. EH is a polygenic disease caused by the interaction of genetic factors and environmental factors. Finding EH-related genes and then clarifying the genetic mechanism of hypertension has become a research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N33/68
Inventor 张奎星朱鼎良何鑫张怡王谷亮黄薇
Owner SHANGHAI INST OF HYPERTENSION