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Method for distinguishing gene types of fast and slow featherings of chicks and method for distinguishing sexes of chicks

A genotype, fast and slow technology, applied in the fields of molecular biology and animal breeding, can solve the problems of identification failure and inability to detect genotypes, and achieve the effects of reliable data, reduced cultivation costs, and simple identification methods.

Active Publication Date: 2014-01-15
BEIJING HUADU YUKOU POULTRY
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Problems solved by technology

At the same time, the trait of slow feathering is dominant to the trait of fast feathering. The existing method of using repeated fragments to identify fast and slow feathering in chicken flocks can only detect phenotypes, but not genotypes, which requires two to three generations to purify
In addition, in some brown shell strains, when identified by repeated fragments or Mbo I digestion, the amplification cannot be effectively performed, resulting in failure of identification

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  • Method for distinguishing gene types of fast and slow featherings of chicks and method for distinguishing sexes of chicks
  • Method for distinguishing gene types of fast and slow featherings of chicks and method for distinguishing sexes of chicks
  • Method for distinguishing gene types of fast and slow featherings of chicks and method for distinguishing sexes of chicks

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with specific embodiments.

[0038] The technical means used in the following examples, unless otherwise specified, can adopt prior art means; the instruments, reagents, etc. used, unless otherwise specified, can be obtained through public channels.

[0039] (1) Primer design and preparation:

[0040] According to the research results under the existing technology, the fast and slow feathers are located in the range of 10.38Mb-11.63Mb (the chicken genome physical map of version 2.1 is 9.86Mb-11.11Mb) (ICGSC Gallus_gallus-4.0 / galGal4, Nov. 2011, UCSC). For the SNP sites in the region, the SNP sites with different performances in fast feathers and slow feathers were obtained, and primers were designed, tested and verified by different individuals.

[0041] Primers were designed using the above-mentioned SNP found in the region around 11Mb on the chicken Z chromosome, and primers were synthesized by Shan...

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Abstract

The invention discloses a method for distinguishing the gene types of fast and slow featherings of chicks and a method for distinguishing the sexes of the chicks. According to the method for distinguishing the gene types, DNA in the blood sample of a chick is subjected to PCR amplification; a used forward primer F is 5'-GCACATTCAAGTAAGCAGTAGTTT-3', and a reverse primer R is 5'-AAAAAAAAAAAAAATTGCACTTTAATAGTACCATCTATTC-3'; an amplified product is subjected to digestion through a TaqI incision enzyme; the lengths of the segments after digestion are detected; the feathering rate gene types of the chick is judged on the basis of the lengths. According to the method for distinguishing the sexes of the chicks, a matching system is established based on the feathering rate gene types obtained through the method for distinguishing the gene types, and the sex of the chick is distinguished according to the characters of the fast and slow featherings. According to the invention, various gene types of fast and slow featherings of the chicks can be effectively distinguished, and the time for establishing the matching system of distinguishing the sexes of the chicks through the feathering rates is greatly shortened.

Description

technical field [0001] The invention relates to a method for identifying chicken fast and slow feather genotypes and a method for identifying male and female chickens (self-separation) under the above-mentioned genotype establishment, and belongs to the technical field of molecular biology and the technical field of animal breeding. Background technique [0002] Sex discrimination plays an important role in modern chicken production. The techniques currently used for male and female identification mainly include anal turning and using feather color, feather speed, etc. to distinguish between sexes. Since the identification of anus turning requires special training for the identification personnel, and it will cause harm to the flock during the identification process. Therefore, in order to provide better quality chicks for laying hen breeders and ensure their economic benefits, a method for chicken flocks is adopted. The technique of sex-separation without injury is particu...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124
Inventor 韩文朋吴桂琴刘爱巧宋海泉石凤英李竞一赵春江
Owner BEIJING HUADU YUKOU POULTRY
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