Molecular marker related to growth of litopenaeus vannamei and application thereof

A technology of molecular markers and vanabin, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of genes or markers that are not directly related to localization and growth, so as to improve efficiency and The effect of accuracy

Active Publication Date: 2017-06-13
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

In prawns, the researchers located several growth-related QTL loci through the QTL mapping method. For example, in the QTL mapping of the growth rate of Penaeus japonica, the researchers located a main effect AFLP marker related to growth traits, and then through the map position Cloning method to find a growth-related gene ELOVL (Elongation of very longchain fatty acids-like) (Lyons RE, Dierens LM, Tan SH, Preston NP, LiY. Characterization of AFLP markers associated with gr...

Method used

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  • Molecular marker related to growth of litopenaeus vannamei and application thereof
  • Molecular marker related to growth of litopenaeus vannamei and application thereof

Examples

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Effect test

Embodiment 1

[0014] Example 1: Obtaining growth-related molecular markers of Litopenaeus vannamei

[0015] (1) Location of growth QTL in Litopenaeus vannamei:

[0016] The material used for QTL mapping is a full-sib family material, which is composed of paternal material, maternal material and corresponding offspring. The material was constructed in Fangchenggang SPF vannamei breeding base of Guangxi Fishery Institute in June 2012. When the offspring grow to about 10cm, 250 offspring were randomly sampled. The weight of the offspring was measured using an electronic balance, and the body length was measured using a ruler and a vernier caliper. , carapace length, width of the third abdominal segment, height of the third abdominal segment, width of the first abdominal segment, and height of the first abdominal segment, and sex determination.

[0017] The plant genome extraction kit (Tiangen, Beijing) was used to extract the DNA of all materials, and then SNP typing was performed on all indi...

Embodiment 2

[0029] Example 2: Application of a growth-related marker of Litopenaeus vannamei in breeding.

[0030] (1) Construction of breeding materials

[0031] The breeding materials were constructed in 2015, and 26 families were selected for M1286-109-T / C marker-assisted selection breeding research. The 26 families were divided into two groups according to the establishment time, and each group contained 13 families. When the family material grew to 3cm, each family material was labeled with VIE fluorescence and mixed cultured. After 3 months of breeding, individuals were randomly selected from the two pools for phenotypic measurement, mainly including body weight, body length, total length, carapace length, carapace width and other traits.

[0032] (2) M1286-109-T / C marking type

[0033] The plant genome extraction kit (Tiangen, Beijing) was used to extract the DNA of the above-mentioned family materials, and the DNA concentration was determined using Nanodrop1000, and then the tar...

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Abstract

The invention belongs to the technical field of aquatic breeding, and particularly relates to a molecular marker related to growth of litopenaeus vannamei and application thereof. The molecular marker is located at the 109bp position of SEQM1286, and is a T/C type mutant; the individual growth speed of a CC gene in the marker is higher than the growth speed of a TT gene, that is to say, the CC gene type is a growth-advantage gene type. The molecular marker can be used as the molecular marker of prawn breeding to assist the genetic breeding of prawns.

Description

technical field [0001] The invention belongs to the field of aquatic animal breeding, and in particular relates to a growth-related molecular marker of Litopenaeus vannamei and an application thereof. Background technique [0002] Litopenaeus vannamei, also known as white Pacific shrimp, is the most productive prawn species in my country and even in the world, and is also an important economic and trade species in the world. Due to its fast growth rate, strong disease resistance, and after desalination, it can be cultivated in inland low-salinity or even freshwater areas. Therefore, this species is widely cultivated in the north and south of my country and coastal inland areas. The annual output of my country is more than 100. tons. [0003] Improved species are the basis and driving force for the healthy development of the Litopenaeus vannamei industry. Artificial breeding is an important method for cultivating improved species of Litopenaeus vannamei with fast growth and h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李富花王全超于洋相建海
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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