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Method for detecting micro-satellite marker LJ_liu of Lateolabrax japonicus

A technique for microsatellite marker and serratia, which is applied in the detection field of the microsatellite marker LJ_liu of serratia and the microsatellite marker LJ_liu of sagebrush, can solve the problems of restricting the research and development of molecular genetics and the like, and achieve the effect of simple method.

Inactive Publication Date: 2009-07-22
OCEAN UNIV OF CHINA
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Problems solved by technology

However, due to the lack of microsatellite markers in perch, the development of its molecular genetics research is limited, so there is an urgent need to obtain microsatellite markers for the research and application of genetic diversity and pedigree authentication of perch

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  • Method for detecting micro-satellite marker LJ_liu of Lateolabrax japonicus

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Embodiment Construction

[0022] The following examples describe in detail the method of the present invention on the microsatellite core sequence DNA molecular genetic marker of the perch LJ_liu. Firstly, the genomic DNA in the blood of the perch was extracted and diluted for use; then, using the microsatellite core sequence contained in the AFLP band of the perch, specific primers were designed at both ends of the sequence; The genomic DNA of individuals in the perch group was amplified by PCR, and the PCR products were detected by denatured polyacrylamide gel electrophoresis to determine the genotype of each individual, that is, to obtain the genetic polymorphism map of perch.

[0023] 1. Extraction of Genomic DNA from Perch: Add 100 μl of perch blood into an Eppendorf tube, then add 500 μl of cell lysate and 5 μl of 20 mg / ml proteinase K, shake gently, and leave overnight at 37°C. Then, 600 μl of phenol chloride: imitation: isoamyl alcohol (25:24:1) mixture was added to the lysed sample, shaken for...

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Abstract

A detection method of microsatellite marker LJ_liu in sea bass. Firstly, the genomic DNA in the blood of the perch was extracted and diluted for use; then, using the microsatellite core sequence contained in the AFLP band of the perch, specific primers were designed at both ends of the sequence; The genomic DNA of individuals in the perch group was amplified by PCR, and the PCR products were detected; the bands that appeared in the products were analyzed to determine the genotype of each individual, and the genetic polymorphism map of the perch was obtained. The present invention is characterized in that the genetic variation map of the perch genetic marker LJ_liu can be quickly obtained, and the method is simple and convenient. Moreover, compared with other molecular genetic marker technologies, microsatellite markers conform to the Mendelian inheritance pattern, showing co-dominant inheritance, and the results obtained can visually detect the genotype of each individual perch; it can be applied to different geographical groups or perch Genetic markers, pedigree certification and construction of genetic maps among populations of C. perch within groups.

Description

technical field [0001] The invention belongs to the DNA molecular genetic marker technology of Lateolabrax japonicus, and relates to a technical method for detecting the Lateolabrax japonicus microsatellite marker LJ_liu, in particular to a detection method of the Lateolabrax japonicus microsatellite marker LJ_liu. Background technique [0002] Lateolabrax japonicus, also known as perch, is a broad-temperate near-shore middle and lower layer fish. It is distributed in the coastal waters of my country, Japan, and North Korea. It is an important species for coastal breeding and export in my country. In order to rationally utilize and protect the germplasm resources of wild perch, some scholars have studied the genetic structure and genetic diversity of perch by using isozyme technology and RAPD technology. However, since both isozyme markers and RAPD markers are dominant genetic markers, the detection efficiency is not as high as co-dominant markers such as microsatellite mark...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 刘云国李八方
Owner OCEAN UNIV OF CHINA
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