Magnetic nano cell automatic sorting device and uses thereof

An automatic sorting and magnetic nano technology, applied in the field of biomedicine, can solve the problems that the sorting system cannot realize automatic sorting, and achieve the effect of reducing the occurrence of GVHD, reducing the occurrence of GVHD, and improving efficiency

Inactive Publication Date: 2009-08-12
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a magnetic nano-cell automatic sorting device and its application to overcome the inability of the existing sorting system to realize automation and the sorting of a large number of cells

Method used

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  • Magnetic nano cell automatic sorting device and uses thereof
  • Magnetic nano cell automatic sorting device and uses thereof
  • Magnetic nano cell automatic sorting device and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Synthesis of Magnetic Nanoparticles

[0043] FeSO 4 ·7H 2 O and FeCl 3 ·6H 2 O mixed solution and NaOH solution form γ-Fe by co-precipitation method 2 o 3 nanoparticles, and then encapsulating chitosan in γ-Fe 2 o 3 outside to form core-shell magnetic nanoparticles and characterized by TEM and Mössbauer spectroscopy.

Embodiment 2

[0045] Anti-CD34 Monoclonal Antibody Linked to Magnetic Nanoparticles

[0046] Disperse a certain amount of the above-mentioned magnetic nanoparticles linked to amino groups in phosphate buffered saline (Phosphatebuffered saline, PBS, 0.1M, pH7.4), add a certain amount of glutaraldehyde, react for 4-5 hours, and remove excess glutaraldehyde Dialdehyde was washed away, redispersed in PBS, and then added a certain amount of anti-CD34 monoclonal antibody, reacted for 1.5-2.5 hours, and then the excess antibody was fully washed with PBS, and the residual liquid was collected at the same time (antibody reacted with magnetic nanoparticles The remaining liquid after washing), two rinses (referred to as the first wash and the second wash).

Embodiment 3

[0048] Morphology of isolated CD34+ cells

[0049] figure 2 is a SEM image of CD34+ cells with magnetic nanoparticles. With the help of SEM, it can be seen that the isolated CD34+ cells still maintain normal morphology, and at the same time we can also see the magnetic nanoparticles attached to the cell surface. From the figure, we can also see that the magnetic nanoparticles are relatively small in size compared to the cells. Although the magnetic nanoparticles have a certain aggregation phenomenon in the complex biological system, it does not affect the separation effect of the cells and does not have much effect on the shape of the cells. Impact. Therefore, isolation of CD34+ cells using magnetic nanoparticles has little effect on cell morphology.

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Abstract

The invention relates to a magnetic nanometer cell automatic sorting device and its application. The device is at least composed of a magnetic sorting system, a sample transmission system and a computer control system. The magnetic sorting system at least includes a magnetic sorting solution, a sorting column and an optional Moving the magnetic field, the magnetic separation solution contains gamma-Fe2O3 magnetic nanoparticles wrapped in chitosan. It can be used to separate hematopoietic stem cells from peripheral blood or umbilical cord blood, and adopts microcomputer and modern control technology to automatically control the whole process of cell sorting and washing. It can separate stem cells in batches, safely, efficiently and quickly, and obtain A large number of high-purity hematopoietic stem cells can be used as a cell source for cytological research, large-throughput drug screening, in vitro culture and animal experiments.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, in particular to a device for cell sorting using magnetic nanoparticles. Background technique [0002] Since the 1980s, hematopoietic stem cell transplantation has become the main measure of hematopoietic support after high-dose radiotherapy and chemotherapy for malignant tumors (such as some malignant hematological diseases and non-hematological solid tumors, etc.). With the support of hematopoietic stem cell transplantation, clinicians can use super-large doses of radiotherapy and chemotherapy to eliminate cancer cells in the patient's body to the greatest extent, and then implant hematopoietic stem cells to rebuild the destroyed hematopoietic and immune systems and improve the survival rate of malignant tumors. Treatment efficacy and patient survival. Since hematopoietic stem cells mainly exist in the bone marrow in adults, hematopoietic stem cells derived from bone marrow are most common...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00C12N5/08
Inventor 沈鹤柏潘建国董亚明朱龙章韩林峰
Owner SHANGHAI NORMAL UNIVERSITY
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