Ferrite/bacille calmette-guerin vaccine/temperature sensing gel integration material and preparation thereof

A technology of thermosensitive gel and BCG, which is applied in the field of biocomposite materials, materials science and biomedicine, can solve problems such as affecting the curative effect, and achieve the effect of prolonging the residence time and enhancing the efficacy of the drug.

Inactive Publication Date: 2009-11-18
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, the residence time of BCG in the bladder is only 1-2 hours, that is, it is excreted with the urine, which seriously affects its curative effect, and it needs multiple infusions after the operation, which brings great pain to the patient.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1) Prepare a volume ratio of 1.75:1 and a concentration of 0.1mol L -1 FeCl 3 and FeSO 4 Solution, under mechanical stirring and passing N 2 Under these conditions, the two solutions were mixed homogeneously. Add 0.1% (gained Fe after theoretical calculation with the volume of the two solutions that are prepared in step 1) 3 o 4 The quality of the standard) surfactant polyethylene glycol-6000 (PEG-6000). After stirring for 15 minutes, add 1 mol·L dropwise to the mixed solution -1 NaOH solution until the solution pH = 9, a black precipitate was obtained. Under 70 ℃ water bath, make it mature. Stand still for 5 hours, remove the supernatant, and repeatedly wash the generated particles with distilled water and absolute ethanol to wash away the surfactant and impurity ions on the surface of the particles. Vacuum dried at 50°C and ground through a 100-mesh sieve to obtain Fe 3 o 4 particles.

[0015] 2) 90mg of chitosan with a degree of deacetylation of 95% was di...

Embodiment 2

[0021] 1) Prepare a volume ratio of 1.75:1 and a concentration of 0.1mol L -1 FeCl 3 and FeSO 4 Solution, under mechanical stirring and passing N 2 Under these conditions, the two solutions were mixed homogeneously. Add 0.5wt% (gained Fe after theoretical calculation with the volume of two kinds of solutions that step 1 is prepared 3 o 4 The quality of the standard) surfactant polyethylene glycol-6000 (PEG-6000). After stirring for 15 minutes, add 1 mol·L dropwise to the mixed solution -1 NaOH solution until the solution pH = 9, a black precipitate was obtained. Under 70 ℃ water bath, make it mature. Stand still for 5 hours, remove the supernatant, and repeatedly wash the generated particles with distilled water and absolute ethanol to wash away the surfactant and impurity ions on the surface of the particles. Vacuum dried at 50°C and ground through a 100-mesh sieve to obtain Fe 3 o 4 particles.

[0022] 2) 80mg of chitosan with a degree of deacetylation of 95% was ...

Embodiment 3

[0028] 1) Prepare a volume ratio of 1.75:1 and a concentration of 0.1mol L -1 FeCl 3 and FeSO 4 Solution, under mechanical stirring and passing N 2 Under these conditions, the two solutions were mixed homogeneously. Add 0.7wt% (gained Fe after theoretical calculation with the volume of two kinds of solutions that step 1 is prepared 3 o 4 The quality of the standard) surfactant polyethylene glycol-6000 (PEG-6000). After stirring for 15 minutes, add 1 mol·L dropwise to the mixed solution -1NaOH solution until the solution pH = 9, a black precipitate was obtained. Under 70 ℃ water bath, make it mature. Stand still for 5 hours, remove the supernatant, and repeatedly wash the generated particles with distilled water and absolute ethanol to wash away the surfactant and impurity ions on the surface of the particles. Vacuum dried at 50°C and ground through a 100-mesh sieve to obtain Fe 3 o 4 particles.

[0029] 2) 70mg of chitosan with a degree of deacetylation of 95% was d...

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PUM

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Abstract

The invention discloses a ferrite / BCG / temperature-sensitive gel integrated material, which comprises 5%-15% of BCG, 5%-15% of ferric oxide, 70%-90% of chitosan / Sodium β-glycerophosphate xerogel. The preparation method of the integrated material is to prepare nano ferric oxide by co-precipitation method; dissolve chitosan in dilute hydrochloric acid, add quantitatively prepared ferric oxide and BCG, disperse evenly, and then add β-sodium glycerophosphate dropwise The solution is heated in a water bath to convert the mixed solution into a gel; the obtained gel is freeze-dried or vacuum-dried to obtain a chitosan xerogel; the xerogel is ground and sieved to obtain a corresponding integrated material. The integrated material prepared by the present invention has the function of targeting wall attachment and sustained release. The integrated material powder can be attached to the inner wall of the bladder under the action of an external magnetic field so as not to be excreted with urine, prolonging the time it stays in the bladder and strengthening the BCG. efficacy.

Description

technical field [0001] The invention relates to a biocomposite material composed of chitosan / beta-glycerophosphate sodium gel as a carrier, ferric iron tetroxide as a targeting material and freeze-dried Bacillus Calmette-Guerin, belonging to the technical field of biocomposite materials intersecting material science and biomedicine. Background technique [0002] At present, 90% to 95% of bladder cancers are transitional epithelial cell carcinomas clinically, and most of them are superficial carcinomas in the early stage of the disease. The treatment of superficial bladder cancer mainly relies on transurethral resection or electrocautery. Hospitals always perform TURBT surgery for this, and this kind of surgery often has residual cancer cells, and the tumor is prone to recurrence. If no treatment is performed after the operation, the recurrence rate can reach 60% to 90%. %. Intravesical infusion of Bacillus Calmette-Guerin (BCG) is currently recognized as the best way to pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/36A61K47/02A61K47/24A61K39/04A61P35/00A61P13/10
Inventor 孙康宁冷亮李爱民吴承格赵琰
Owner SHANDONG UNIV
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